Optimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor-caju

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dc.contributor.authorPatrick, Ferdinandi
dc.contributor.authorMtui, Godliving
dc.contributor.authorMligo, Anthony M.
dc.contributor.authorKivaisi, Amelia K.
dc.date.accessioned2016-05-17T13:28:53Z
dc.date.available2016-05-17T13:28:53Z
dc.date.issued2013
dc.description.abstractA white-rot fungus, Pleurotus sajor-caju, was isolated from coastal Tanzania and screened for crude lignolytic enzymes production using rhemazol brilliant blue R (RBBR) dye, 2,2-azino-bis (3- ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Laccase (Lac) and manganese peroxidase (MnP) were detected by α-napthol and pyrogallol solutions, respectively, on the guaiacol supplemented semi-solid media. The effect of temperature, pH, carbon, nitrogen, Cu2+, 2,5- xylidine, ferulic acid, Mn2+ and immobilization using Luffa cylindrica sponges in submerged culture fermentations were investigated for maximum enzymes production. After 7 days of incubation, 83 to 100% oxidation of RBBR, ABTS and guaiacol was observed. With optimized culture conditions, the fungal filtrate had maximum Lac and MnP activities of 80 and 0.94 U/ml, respectively compared to 0.62 and 0.0003 U/ml obtained with non-optimized ones; amounting to 129 and 3133 times increase in Lac and MnP activities, respectively. The improved crude enzymes activities, RBBR decolourization, ABTS and guaiacol oxidation capabilities of P. sajor-caju show its potential as a source of industrial enzymes for biotechnological applications.en_US
dc.identifier.citationPatrick, F., Mtui, G., Mshandete, A.M. and Kivaisi, A., 2013. Optimization of laccase and manganese peroxidase production in submerged culture of Pleurotus sajorcaju. African Journal of Biotechnology, 10(50), pp.10166-10177.en_US
dc.identifier.doi10.5897/AJB11.863
dc.identifier.issn1684–5315
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2096
dc.language.isoenen_US
dc.subjectWhite-rot fungien_US
dc.subjectOptimizationen_US
dc.subjectPleurotus sajor-cajuen_US
dc.subjectLaccaseen_US
dc.subjectManganese peroxidaseen_US
dc.subjectSubmerged fermentationen_US
dc.subjectImmobilizationen_US
dc.titleOptimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor-cajuen_US
dc.typeJournal Article, Peer Revieweden_US
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