Optimization of Laccase and Manganese Peroxidase Production in Submerged Culture of Pleurotus Sajor-caju
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Date
2013
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Abstract
A white-rot fungus, Pleurotus sajor-caju, was isolated from coastal Tanzania and screened for crude
lignolytic enzymes production using rhemazol brilliant blue R (RBBR) dye, 2,2-azino-bis (3-
ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Laccase (Lac) and
manganese peroxidase (MnP) were detected by α-napthol and pyrogallol solutions, respectively, on the
guaiacol supplemented semi-solid media. The effect of temperature, pH, carbon, nitrogen, Cu2+, 2,5-
xylidine, ferulic acid, Mn2+ and immobilization using Luffa cylindrica sponges in submerged culture
fermentations were investigated for maximum enzymes production. After 7 days of incubation, 83 to
100% oxidation of RBBR, ABTS and guaiacol was observed. With optimized culture conditions, the
fungal filtrate had maximum Lac and MnP activities of 80 and 0.94 U/ml, respectively compared to 0.62
and 0.0003 U/ml obtained with non-optimized ones; amounting to 129 and 3133 times increase in Lac
and MnP activities, respectively. The improved crude enzymes activities, RBBR decolourization, ABTS
and guaiacol oxidation capabilities of P. sajor-caju show its potential as a source of industrial enzymes
for biotechnological applications.
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Keywords
White-rot fungi, Optimization, Pleurotus sajor-caju, Laccase, Manganese peroxidase, Submerged fermentation, Immobilization
Citation
Patrick, F., Mtui, G., Mshandete, A.M. and Kivaisi, A., 2013. Optimization of laccase and manganese peroxidase production in submerged culture of Pleurotus sajorcaju. African Journal of Biotechnology, 10(50), pp.10166-10177.