High fat diet worsens the adverse effects of antibiotic on intestinal health in juvenile Nile tilapia (Oreochromis niloticus).
Loading...
Date
2019-08-25
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Antibiotics and high fat diets are commonly used independently in global aquaculture production for fish health
management and to spare the use of costly protein as energy sources, respectively, causing relatively similar meta bolic effects and stresses. However, it is unknown whether dietary high fat worsens or attenuates the adverse effects
caused by antibiotics in fish. We determined the ability of high fat diet to influence the adverse effects of oxytetra cycline on Nile tilapia, Oreochromis niloticus. Thirty Nile tilapia weighing 8.45 ± 0.15 g were fed on medium fat (MF;
70 g/kg) and high fat (HF; 120 g/kg) diets and the same fat levels supplemented with 2.00 g/kg diet of OTC
(80 mg/kg body weight/day) hereafter, MFO and HFO for 65 days. The general growth performance, feed efficiency
and intestinal health of fish were evaluated. The Nile tilapia fed on HFO diet had significantly lower growth rate,
body protein content and feed efficiency compared to those fed on MFO diet. Dietary HFO affected the intestine
histomorphology, which decreased dramatically the tight junction proteins of Nile tilapia and induced microbiota
dysbiosis compared to MFO diet. The Nile tilapia fed on HFO diet had increased oxidative stress, which stimulated
drug detoxification response, caused endoplasmic reticulum stress and apoptosis compared to those fed on MFO
diet. The new findings from our study demonstrate that, the adverse effects of antibiotics in fish are different at me dium and high fat contents. Feeding fish with high fat diets with antibiotics worsen the adverse effects. This en lightens our understanding on the risks of antibiotics misuse and also suggests that antibiotics should be more
strictly limited in aquaculture, in which high fat diets are currently widely used in fish production worldwide.
Description
Keywords
Oxytetracycline, High fat diets with antibiotics, Microbiota dysbiosis, Oxidative stress, Endoplasmic reticulum stress, Apoptosis