Improving propagation success of Dalbegia melanoxylon (African blackwood) in Tanzania (I): Characterization of mycorrhizal associated with D. melanoxylon (African blackwood) in Tanzania

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dc.contributor.authorWasha, W. B. A.
dc.contributor.authorNyomora, Agnes M. S.
dc.contributor.authorLyaruu, H. M. V.
dc.date.accessioned2016-05-05T14:12:04Z
dc.date.available2016-05-05T14:12:04Z
dc.date.issued2012
dc.description.abstractDalbergia melanoxylon Guill & Perr is a plant with quality wood in the World and therefore is over harvested for timber while its regeneration capacity is very low. The propagation techniques such as tissue culture or use of mycorrhiza have not been investigated which instigated conduction of this study by investigating the presence of mycorrhiza that might be useful for its regeneration. Some 120kg of soil and 120 roots of D. melanoxylon from Kilwa, Kilosa and Babati were sampled to identify mycorrhiza related with regeneration of the species. Cleaning the tissues was done by soaking the root pieces in 1.79M KOH, and 0.1M Hcl. Staining was done using 0.05% Trypan blue and de-staining in 14:1:1 lactic acid: glycerol: water and mounting on slides for observation. Soil was soaked in water overnight and the mycorrhiza was separated using a stereo microscope at 50X. Separated mycorrhiza were incubated for observation using a compound microscope at 400X. Twenty six (26) ECM resembling Inocybe and Laccaria sp were isolated from the root pieces and 18 VAM resembling Glomus species were isolated from soil. It is recommended that future research should investigate proper inoculum types and time for inoculating the species in nurseries for propagationen_US
dc.identifier.citationWasha, W.B.A., Nyomora, M.S. and Lyaruu, H.V.M., 2012. Improving propagation success of Dalbegia melanoxylon (African blackwood) in Tanzania (I): Characterization of mycorrhizal associated with D. melanoxylon (African blackwood) in Tanzania. Tanzania Journal of Science, 38(1), pp.35-42.en_US
dc.identifier.issn0856 1761
dc.identifier.urihttp://hdl.handle.net/20.500.11810/1846
dc.language.isoenen_US
dc.subjectDalbergia melanoxylonen_US
dc.subjectEctomycorrhizaen_US
dc.subjectEndomycorrhizaen_US
dc.subjectMycorrhizaen_US
dc.titleImproving propagation success of Dalbegia melanoxylon (African blackwood) in Tanzania (I): Characterization of mycorrhizal associated with D. melanoxylon (African blackwood) in Tanzaniaen_US
dc.typeJournal Article, Peer Revieweden_US
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