Improving propagation success of Dalbegia melanoxylon (African blackwood) in Tanzania (I): Characterization of mycorrhizal associated with D. melanoxylon (African blackwood) in Tanzania
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Date
2012
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Abstract
Dalbergia melanoxylon Guill & Perr is a plant with quality wood in the World and therefore is
over harvested for timber while its regeneration capacity is very low. The propagation techniques
such as tissue culture or use of mycorrhiza have not been investigated which instigated conduction
of this study by investigating the presence of mycorrhiza that might be useful for its regeneration.
Some 120kg of soil and 120 roots of D. melanoxylon from Kilwa, Kilosa and Babati were sampled
to identify mycorrhiza related with regeneration of the species. Cleaning the tissues was done by
soaking the root pieces in 1.79M KOH, and 0.1M Hcl. Staining was done using 0.05% Trypan
blue and de-staining in 14:1:1 lactic acid: glycerol: water and mounting on slides for observation.
Soil was soaked in water overnight and the mycorrhiza was separated using a stereo microscope
at 50X. Separated mycorrhiza were incubated for observation using a compound microscope at
400X. Twenty six (26) ECM resembling Inocybe and Laccaria sp were isolated from the root
pieces and 18 VAM resembling Glomus species were isolated from soil. It is recommended that
future research should investigate proper inoculum types and time for inoculating the species in
nurseries for propagation
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Keywords
Dalbergia melanoxylon, Ectomycorrhiza, Endomycorrhiza, Mycorrhiza
Citation
Washa, W.B.A., Nyomora, M.S. and Lyaruu, H.V.M., 2012. Improving propagation success of Dalbegia melanoxylon (African blackwood) in Tanzania (I): Characterization of mycorrhizal associated with D. melanoxylon (African blackwood) in Tanzania. Tanzania Journal of Science, 38(1), pp.35-42.