Inhibition of lipophagy suppresses lipid metabolism in zebrafish liver cells, Frontiers in Physiology, 10; Article 1077; 1-9.

Simple item page
dc.contributor.authorJing Wang
dc.contributor.authorSi-Lan Han
dc.contributor.authorDong-Liang Lu
dc.contributor.authorLing-Yu Li
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorDongliang Li
dc.contributor.authorMeiling Zhang
dc.contributor.authorZhen-Yu Du
dc.date.accessioned2021-04-18T05:24:47Z
dc.date.available2021-04-18T05:24:47Z
dc.date.issued2019-08-21
dc.description.abstractLipophagy degrades lipid droplets (LDs) through the lysosomal degradative pathway, thus plays important roles in regulating lipid metabolism in mammals. However, information on the existence and functions of lipophagy in fish lipid metabolism is still limited. In the present study, we confirmed the existence of lipophagy by observing the structures of LDs sequestered in autophagic vacuoles in the zebrafish liver cell line (ZFL) via electronic microscopy. Moreover, starved cells increased the mRNA expression of the microtubule-associated protein 1A/1B light chain 3 beta (LC3), which is a marker protein for autophagy and protein conversion from LC3-I to LC3-II. Inhibiting autophagy with chloroquine increased significantly the LDs content and decreased fatty acid β-oxidation and esterification activities in the ZFL cells cultured in the fed state. Furthermore, inhibiting autophagy function downregulated the mRNA expression of the genes and their proteins related to lipid metabolism. Altogether, the present study verified the existence of lipophagy and its essential regulatory roles in lipid metabolism in fish cells.en_US
dc.description.sponsorshipThe National Natural Science Foundation of China (31830102) and Program of Shanghai Academic Research Leader (19XD1421200).en_US
dc.identifier.citationJing Wang, Si-Lan Han, Dong-Liang Lu, Ling-Yu Li, Samwel Mchele Limbu, Dongliang Li, Meiling Zhang and Zhen-Yu Du (2019). Inhibition of lipophagy suppresses lipid metabolism in zebrafish liver cells, Frontiers in Physiology, 10; Article 1077; 1-9. https://doi.org/10.3389/fphys.2019.01077en_US
dc.identifier.doihttps://doi.org/10.3389/fphys.2019.01077
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5605
dc.publisherFrontiers Media SAen_US
dc.subjectzebrafish; liver cells; lipophagy; lipid metabolism; fatty acid β-oxidation; esterificationen_US
dc.titleInhibition of lipophagy suppresses lipid metabolism in zebrafish liver cells, Frontiers in Physiology, 10; Article 1077; 1-9.en_US
dc.typeJournal Article, Peer Revieweden_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Inhibited Carnitine Synthesis Causes .pdf
Size:
1.97 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Department of Aquaculture Technology