Inhibition of lipophagy suppresses lipid metabolism in zebrafish liver cells, Frontiers in Physiology, 10; Article 1077; 1-9.
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Date
2019-08-21
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media SA
Abstract
Lipophagy degrades lipid droplets (LDs) through the lysosomal degradative pathway,
thus plays important roles in regulating lipid metabolism in mammals. However,
information on the existence and functions of lipophagy in fish lipid metabolism is still
limited. In the present study, we confirmed the existence of lipophagy by observing the
structures of LDs sequestered in autophagic vacuoles in the zebrafish liver cell line (ZFL)
via electronic microscopy. Moreover, starved cells increased the mRNA expression of the
microtubule-associated protein 1A/1B light chain 3 beta (LC3), which is a marker protein
for autophagy and protein conversion from LC3-I to LC3-II. Inhibiting autophagy with
chloroquine increased significantly the LDs content and decreased fatty acid β-oxidation
and esterification activities in the ZFL cells cultured in the fed state. Furthermore,
inhibiting autophagy function downregulated the mRNA expression of the genes and
their proteins related to lipid metabolism. Altogether, the present study verified the
existence of lipophagy and its essential regulatory roles in lipid metabolism in fish cells.
Description
Keywords
zebrafish; liver cells; lipophagy; lipid metabolism; fatty acid β-oxidation; esterification
Citation
Jing Wang, Si-Lan Han, Dong-Liang Lu, Ling-Yu Li, Samwel Mchele Limbu, Dongliang Li, Meiling Zhang and Zhen-Yu Du (2019). Inhibition of lipophagy suppresses lipid metabolism in zebrafish liver cells, Frontiers in Physiology, 10; Article 1077; 1-9. https://doi.org/10.3389/fphys.2019.01077