Dietary thiamine modulates carbohydrate metabolism, antioxidant status, and alleviates hypoxia stress in oriental river prawn Macrobrachium nipponense (de Haan)

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dc.contributor.authorDongsheng Zhou
dc.contributor.authorChengli Wang
dc.contributor.authorJinxian Zheng
dc.contributor.authorJianhu Zhao
dc.contributor.authorShanshan Wei
dc.contributor.authorYunfeng Xiong
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorYouqin Kong
dc.contributor.authorFang Cao
dc.contributor.authorZhili Ding
dc.date.accessioned2023-04-14T14:19:53Z
dc.date.available2023-04-14T14:19:53Z
dc.date.issued2022-12
dc.description.abstractHypoxia is one of the challenges in prawns aquaculture. However, the role of thiamine, which is a coenzyme in carbohydrate metabolism with antioxidant properties, in reducing hypoxia in prawns aquaculture is currently unknown. We investigated the effects of thiamine on antioxidant status, carbohydrate metabolism and acute hypoxia in oriental river prawn, Macrobrachium nipponense. One thousand eight hundred prawns (0.123 ± 0.003 g) were fed five diets (60 prawns each tank, six replicates per diet) supplemented with graded thiamine levels (5.69, 70.70, 133.67, 268.33 and 532.00 mg/kg dry mater) for eight weeks and then exposed to hypoxia stress for 12 h followed by reoxyegnation for 12 h. The results showed that, under normoxia, prawns fed the 133.67 or 268.33 mg/kg thiamine diet had significantly lower glucose 6-phosphatedehydrogenase, succinate dehydrogenase and phosphoenolpyruvate carboxykinase activities than those fed the other diets. Moreover, total antioxidant capacity (T-AOC) increased significantly when prawns were fed the 133.67 mg/kg thiamine diet. Superoxide dismutase (SOD) activity and malonaldehyde (MDA) content also increased significantly when prawns were fed the 268.33 or 532.00 mg/kg thiamine diet under hypoxia. And the significantly increased SOD activity and MDA level also observed in prawns fed 532.00 mg/kg thiamine under reoxygenation. Under normoxia, prawns fed the 70.70 or 133.67 mg/kg thiamine diet decreased the mRNA expressions of AMP-activated protein kinase-alpha (AMPK-α), pyruvate dehydrogenase-E1-α subunit (PDH-E1-α) and hypoxia-inducible factor-1s (HIF-1α, HIF-1β), but increased the mRNA expressions of phosphofructokinase (PFK) significantly. After 12 h of hypoxia, the energy metabolism related genes (AMPK-β, AMPK-γ, PFK, PDH-E1-α), hypoxia-inducible factor related genes (HIF-1α, HIF-1β) and thiamine transporter gene (SLC19A2) were up-regulated significantly in prawns fed the 133.67 or 268.33 mg/kg thiamine diets. After 12 h of reoxygenation, prawns fed the 133.67 or 268.33 mg/kg diet significantly decreased the SOD activity, MDA level and SLC19A2 mRNA expression compared with other diets. The optimum thiamine was 161.20 mg/kg for minimum MDA content and 143.17 mg/kg for maximum T-AOC activity based on cubic regression analysis. In summary, supplementing 143.17 to 161.20 mg/kg thiamine in the diets for M. nipponense improves the antioxidant capacity under normoxia and reduces the oxidative damage under hypoxia stress.en_US
dc.description.sponsorshipZhejiang Province Public Welfare Technology Application Research Projecten_US
dc.identifier.citationDongsheng Zhou, Chengli Wang, Jinxian Zheng, Jianhu Zhao, Shanshan Wei, Yunfeng Xiong, Samwel Mchele Limbu, Youqin Kong, Fang Cao and Zhili Ding (2022). Dietary thiamine modulates carbohydrate metabolism, antioxidant status, and alleviates hypoxia stress in oriental river prawn Macrobrachium nipponense (de Haan). Fish & Shellfish Immunology, 131: 42-53. https://doi.org/10.1016/j.fsi.2022.09.059en_US
dc.identifier.doihttps://doi.org/10.1016/j.fsi.2022.09.059
dc.identifier.urihttp://hdl.handle.net/20.500.11810/6032
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectThiamineen_US
dc.subjectMacrobrachium nipponenseen_US
dc.subjectHypoxia stressen_US
dc.subjectAntioxidant capacityen_US
dc.titleDietary thiamine modulates carbohydrate metabolism, antioxidant status, and alleviates hypoxia stress in oriental river prawn Macrobrachium nipponense (de Haan)en_US
dc.typeJournal Article, Peer Revieweden_US
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