Purification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania

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dc.contributor.authorPatrick, Ferdinandi
dc.contributor.authorMtui, Godliving
dc.contributor.authorMshandete, Anthony M.
dc.contributor.authorJohansson, Gunnar
dc.contributor.authorKivaisi, Amelia K.
dc.date.accessioned2016-05-17T13:31:23Z
dc.date.available2016-05-17T13:31:23Z
dc.date.issued2009
dc.description.abstractA lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6- dimethoxyphenol as a substrate.en_US
dc.identifier.citationPatrick, F., Mtui, G., Mshandete, A.M., Johansson, G. and Kivaisi, A., 2009. Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res, 3(5), pp.250-258.en_US
dc.identifier.issn1996-0778
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2112
dc.language.isoenen_US
dc.subjectFunalia trogiien_US
dc.subjectLaccaseen_US
dc.subjectAnion exchange chromatographyen_US
dc.subjectSize exclusion chromatographyen_US
dc.subjectElectrophoresisen_US
dc.subjectIsoelectric focusingen_US
dc.titlePurification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzaniaen_US
dc.typeJournal Article, Peer Revieweden_US
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