Purification and Characterization of a Laccase from the Basidiomycete Funalia Trogii (Berk.) Isolated in Tanzania
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Date
2009
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Abstract
A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal
Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete
rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and
guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593
U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion
chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a
molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH
and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6-
dimethoxyphenol as a substrate.
Description
Keywords
Funalia trogii, Laccase, Anion exchange chromatography, Size exclusion chromatography, Electrophoresis, Isoelectric focusing
Citation
Patrick, F., Mtui, G., Mshandete, A.M., Johansson, G. and Kivaisi, A., 2009. Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res, 3(5), pp.250-258.