Molecular Phylogeny of Saprophytic Wild Edible Mushroom Species from Tanzania Based on ITS and nLSU rDNA Sequences
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Date
2014
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Abstract
many different saprophytic wild edible mushrooms (SWEM). Few studies have been carried
out on characterizing these mushrooms, and those have used conventional methods whereby taxa
were characterized using micro- and macromorphological features which are subtle and sometime
fail to delimit closely related taxa. In this study, eight SWEM taxa were characterized using two
molecular markers—the Internal transcribed spacer (ITS) and the nuclear large subunit (nLSU). The
studied sequences were analyzed together with an additional of 19 GenBank sequences of related
taxa in the genera Lentinus, Polyporus, Panus, Macrolepiota and Auricularia with maximum
likelihood and Aspergillus niger as an outgroup. The BLAST search results on the NCBI database
showed that the studied SWEM have ≥ 92% identity for ITS and ≥ 97 % identity for LSU. The
phylogenetic tree constructed using the ITS data set revealed two major distinct clades with
bootstrap support of 77% and 90% and five sub-clades supporting the five genera. The bootstrap
support were 94% for Lentinus, 100% for Polyporus, 98% for Panus, 98% for Macrolepiota and 90
% for Auricularia, while the nLSU data set revealed the same two major distinct clades but with
higher bootstrap support of 91% and 100%. The five subclades again supporting the five genera were
100% for Lentinus, 100% for Pluteus, 100% for Panus, 99% for Macrolepiota and 100% for
Auricularia. From these results, it is clear that both ITS and LSU delineated the SWEM taxa to the
six genera. However, the obtained support values showed that ITS sequences have the highest
possibility of successful delineating the studied SWEM to species level than LSU. Moreover, the
result also showed the genus Panus forming a monopyletic clade with Lentinus and Polyporus, thus
contributing towards a better understanding of its problematic taxonomic ambiguities.
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Keywords
Indigenous forest, ITS, LSU, Saprophytic, SWEM
Citation
Hussein, J.M., Tibuhwa, D.D., Mshandete, A.M. and Kivaisi, A.K., 2014. Molecular phylogeny of saprophytic wild edible mushroom species from Tanzania based on ITS and nLSU rDNA sequences. Curr. Res. Environ. Appl. Mycol, 4, pp.250-260.