A Rapid PCR‐RFLP Method for Monitoring Genetic Variation among Commercial Mushroom Species*
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Date
2004
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
We report the development of a simplified procedure for restriction fragment length polymorphism (RFLP)
analysis of mushrooms. We have adapted standard molecular techniques to be amenable to an undergraduate
laboratory setting in order to allow students to explore basic questions about fungal diversity and
relatedness among mushroom species. The streamlined protocols allowed students to practice important
molecular techniques within the context of self-designed investigative projects. This laboratory experience
provided opportunities for students to practice strategies for examining molecular diversity among species.
Description
Keywords
RFLP analysis, Biotechnology education, Phylogenetic analysis, Phylogenetic analysis, Mushrooms
Citation
Martin, P., Muruke, M., Hosea, K., Kivaisi, A., Zerwas, N. and Bauerle, C., 2004. A rapid PCR‐RFLP method for monitoring genetic variation among commercial mushroom species*. Biochemistry and Molecular Biology Education, 32(6), pp.390-394.