Larvicidal Efficacy of Cryptomeria Japonica Leaf Essential Oils against Anopheles Gambiae
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central
Abstract
Alternative insecticidal compounds with mortality effect against mosquito life cycle stages are
currently needed. The compounds should be biodegradable and nontoxic to non-targeted insects. Plant based
larvicides provide effective control of vector populations. This study explored Cryptomeria japonica leaf essential oil
larvicidal potency against Anopheles gambiae sensu stricto.
Essential oils (12.5 to 200 μg/mL) extracted from C. japonica leaves were evaluated against An. gambiae s.
s. larvae in both the laboratory and semi field in 6 replicates for each dose. Larval mortality readings were taken at
12, 24, 48, and 72 h post treatment.
C. japonica leaf essential oil yield was 17.06 ± 0.56 mL/kg and 1.60 ± 0.33% (w/w). GC-FID and GC-MS
analyses revealed 22 constituents. Essential oil was more effective against An. gambiae s.s. larvae in the laboratory
than in semi field trials. Mortality increased with increasing dosages (12.5 to 200 μg/mL) in the laboratory (31.75 to
100%) and semi field trials (17.75 to 99.5%), respectively. The LC50 value ranged from 5.55 to 63.92 μg/mL in the
laboratory, and 8.22 to 134.84 μg/mL in semi field conditions, LC90 value ranged from 41.34 to 205.93 μg/mL in the
laboratory and 50.92 to 213.11 μg/mL in semi field conditions.
This study has demonstrated the potential of C. japonica leaf essential oil to cause mortality effects to
An. gambiae s. s. larval populations, however, further studies need to be conducted under field conditions and also
with individual active compounds of C. japonica essential oil.
Description
Keywords
Cryptomeria japonica, Larvicidal efficacy, Anopheles gambiae s.s, Mortality, Essential oil
Citation
Mdoe, F.P., Cheng, S.S., Lyaruu, L., Nkwengulila, G., Chang, S.T. and Kweka, E.J., 2014. Larvicidal efficacy of Cryptomeria japonica leaf essential oils against Anopheles gambiae. Parasites & vectors, 7(1), p.426.