In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites

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dc.contributor.authorRwegasila, Edward
dc.contributor.authorMunissi, Joan J.E
dc.contributor.authorMubofu, Egid B.
dc.contributor.authorNyandoro, Stephen S.
dc.contributor.authorErasto, Paul
dc.date.accessioned2018-11-08T10:09:52Z
dc.date.available2018-11-08T10:09:52Z
dc.date.issued2018-06
dc.description.abstractChitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA in the initial six hours followed by a slow and maintained release up to 72 h. The in vivo antimycobacterial acitivities of both TA and TA-CS/TPP nanocomposites against Mycobacterium indicus pranii (MIP) employing Galleria mellonella larvae as an infection model were evaluated. TA-CS/TPP nanocomposite formulations exhibited remarkable effectiveness against MIP than free TAen_US
dc.description.sponsorshipInternational Foundation for Science (IFS)en_US
dc.identifier.citationEdward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro and Paul Erasto, In vivo antimycobacterial studies of toussaintine A-chitosan nanocomposites, Tanzania Journal of Science. 2018, 44 (2), 16 - 26.en_US
dc.identifier.issn0856-1761
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4965
dc.language.isoenen_US
dc.publisherDar es Salaam University Pressen_US
dc.subjectToussaintine A; chitosan; nanocomposites, antimycobacterial; Galleria mellonellaen_US
dc.titleIn vivo antimycobacterial studies of toussaintine A-chitosan nanocompositesen_US
dc.typeJournal Articleen_US
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