Diurnal Expression of Hetr and Diazocyte Development in the Filamentous Non-Heterocystous Cyanobacterium Trichodesmium Erythraeum

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dc.contributor.authorEl-Shehawy, Rehab
dc.contributor.authorLugomela, Charles
dc.contributor.authorErnst, A.
dc.contributor.authorBergman, Birgitta
dc.date.accessioned2016-06-19T21:28:58Z
dc.date.available2016-06-19T21:28:58Z
dc.date.issued2003
dc.description.abstractThe marine non-heterocystous cyanobacterium Trichodesmium fixes atmospheric N2 aerobically in light. In situ immunolocalization/light microscopy of NifH revealed that lighter, non-granulated cell regions observed correspond to the nitrogenase-containing diazocyte clusters in Trichodesmium IMS101. The number of diazocyte clusters per trichome varied from 0 to 4 depending on trichome length. The constant percentage of diazocytes (approx. 15 %) in cultured strains and five natural populations suggests a developmentally regulated differentiation process. Real-time RT-PCR showed that ntcA, encoding the global nitrogen regulator in cyanobacteria, and hetR, the key regulatory gene in heterocyst differentiation, are both constitutively expressed during a 12 h/12 h light/dark cycle. hetR in addition showed a distinct peak in the dark (close to midnight) while nifH expression commenced 6–8 h later. The expression of all three genes was negatively affected by addition of ammonia. Some early heterocyst differentiation genes were also identified in the genome of Trichodesmium. The data suggest that hetR and ntcA may be required for development and function of diazocytes in Trichodesmium.en_US
dc.identifier.citationEl-Shehawy, R., Lugomela, C., Ernst, A. and Bergman, B., 2003. Diurnal expression of hetR and diazocyte development in the filamentous non-heterocystous cyanobacterium Trichodesmium erythraeum. Microbiology, 149(5), pp.1139-1146.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2610
dc.language.isoenen_US
dc.titleDiurnal Expression of Hetr and Diazocyte Development in the Filamentous Non-Heterocystous Cyanobacterium Trichodesmium Erythraeumen_US
dc.typeJournal Article, Peer Revieweden_US
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