Vitellogenin 1 is essential for fish reproduction by transporting DHA-contained phosphatidylcholine from liver to ovary

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dc.contributor.authorSheng-Xiang Sun
dc.contributor.authorYi-Chan Liu
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorDong-Liang Li
dc.contributor.authorLi-Qiao Chen
dc.contributor.authorMei-Ling Zhang
dc.contributor.authorZhan Yin
dc.contributor.authorZhen-Yu Du
dc.date.accessioned2023-04-14T15:22:22Z
dc.date.available2023-04-14T15:22:22Z
dc.date.issued2023-04
dc.description.abstractVitellogenins (Vtgs) are essential for female reproduction in oviparous animals, yet the exact roles and mechanisms remain unknown. In the present study, we knocked out vtg1, which is the most abundant Vtg in zebrafish, Danio rerio via the CRISPR/Cas 9 technology. We aimed to identify the roles of Vtg1 and related mechanisms in reproduction and development. We found that, the Vtg1-deficient female zebrafish reduced gonadosomatic index, egg production, yolk granules and mature follicles in ovary compared to the wide type (WT). Moreover, the Vtg1-deficient zebrafish diminished hatching rates, cumulative survival rate, swimming capacity and food intake, but increased malformation rate, and delayed swim bladder development during embryo and early-larval phases. The Vtg1-deficiency in female broodstock inhibited docosahexaenoic acid-enriched phosphatidylcholine (DHA-PC) transportation from liver to ovary, which lowered DHA-PC content in ovary and offspring during larval stage. However, the Vtg1-deficient zebrafish increased gradually the total DHA-PC content via exogeneous food intake, and the differences in swimming capacity and food intake returned to normal as they matured. Furthermore, supplementing Vtg1-deficient zebrafish with dietary PC and DHA partly ameliorated the impaired female reproductive capacity and larval development during early phases. This study indicates that, DHA and PC carried by Vtg1 are crucial for female fecundity, and affect embryo and larval development through maternal-nutrition effects. This is the first study elucidating the nutrient and physiological functions of Vtg1 and the underlying biochemical mechanisms in fish reproduction and development.en_US
dc.description.sponsorshipNational Key Research and Develop ment Program (2018YFD0900400)en_US
dc.identifier.citationSheng-Xiang Sun, Yi-Chan Liu, Samwel M. Limbu, Dong-Liang Li, Li-Qiao Chen, Mei-Ling Zhang, Zhan Yin and Zhen-Yu Du (2023). Vitellogenin 1 is essential for fish reproduction by transporting DHA-contained phosphatidylcholine from liver to ovary. BBA-Molecular and Cell Biology of Lipids, 159289. https://doi.org/10.1016/j.bbalip.2023.159289.en_US
dc.identifier.doihttps://doi.org/10.1016/j.bbalip.2023.159289
dc.identifier.urihttp://hdl.handle.net/20.500.11810/6038
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectCRISPR/Cas 9en_US
dc.subjectVitellogeninsen_US
dc.subjectZebrafishen_US
dc.subjectReproductionen_US
dc.subjectDocosahexaenoic aciden_US
dc.titleVitellogenin 1 is essential for fish reproduction by transporting DHA-contained phosphatidylcholine from liver to ovaryen_US
dc.typeJournal Article, Peer Revieweden_US
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