Boron Determination in Biological Materials by Inductively Coupled Plasma Atomic Emission and Mass Spectrometry: Effects of Sample Dissolution Methods

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dc.contributor.authorNyomora, Agnes M. S.
dc.contributor.authorSah, R. N.
dc.contributor.authorBrown, Patrick H.
dc.contributor.authorMiller, R. O.
dc.date.accessioned2016-05-06T14:17:49Z
dc.date.available2016-05-06T14:17:49Z
dc.date.issued1997
dc.description.abstractThis study compares four sample dissolution methods for Boron determination in two National Institute of Standard and Technology (NIST) botanical Standard Reference Materials (SRMs) and three Agriculture Canada/NIST RMs, each having a reference (certified or best estimate) B concentration. The dissolution treatments consisted of: 1) dry ashing at 500° C, 2) wet digestion with HNO3 + H2O2, 3) extraction with hot HNO3 and 4) closed vessel microwave dissolution. The samples were spiked before and after imposing dissolution treatments to study B recovery by inductively coupled plasma mass spectrometric (ICP-MS) analysis. Microwave digests of NIST SRM 1515 and some in-house RMs were also used to compare the B values of ICP-MS and ICP-AES (atomic emission spectrometry). While all three digestion methods (dry ashing, wet ashing and microwave) dissolved botanical samples, only the microwave method worked well for animal tissues. In terms of B values in these materials, there was no significant difference among the three digestion treatments. Near 100% recovery of B spiked before and after the sample dissolution indicates that there may not be a significant loss of B during the dissolution process used in this study. Extraction with hot HNO3 was as effective as the three digestion treatments, and B values for this method agreed well with reference values. For the botanical materials studied, the B values determined by ICP-AES were not significantly different from ICP-MS values. This study shows that a simple, time and labor efficient hot HNO3 extraction is as effective as other digestion/dissolution methods for quantitative B recovery from biological materials.en_US
dc.identifier.citationNyomora, A.M.S., Sah, R.N., Brown, P.H. and Miller, R.O., 1997. Boron determination in biological materials by inductively coupled plasma atomic emission and mass spectrometry: effects of sample dissolution methods. Fresenius' journal of analytical chemistry, 357(8), pp.1185-1191.en_US
dc.identifier.doi10.1007/s002160050328
dc.identifier.issn1432-1130
dc.identifier.urihttp://hdl.handle.net/20.500.11810/1886
dc.language.isoenen_US
dc.publisherSpringer Linken_US
dc.subjectBoron determinationen_US
dc.subjectBoronen_US
dc.titleBoron Determination in Biological Materials by Inductively Coupled Plasma Atomic Emission and Mass Spectrometry: Effects of Sample Dissolution Methodsen_US
dc.typeJournal Article, Peer Revieweden_US
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