Characterization of the Biochemical Pathway of Apoptosis Induced by Three Dglucopyranoside Derivatives from Tulbaghia Violacea
Loading...
Date
2014-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aim: Chinese hamster ovary (CHO) cells were used to identify the most likely apoptotic
biochemical pathway induced by Methyl-α-D-glucopyranoside (MDG), D-fructofuranose- β(2→6)-methyl-α-D-glucopyranoside (DFMDG) and β-D-fructofuranosyl-(2→6)-α-D- glucopyranoside (DFDG) treatments isolated from wild garlic (Tulbaghia violacea).
Methodology: Assays, which detect different morphological and biochemical changes in
the CHO cells were used to examine the apoptosis induction by individual agents, paying
special attention to the mitochondria membrane potential (∆ψm) depolarization.
Results: The major events of apoptosis, namely, translocation of phosphatidylserine,
mitochondria membrane potential (∆ψm) depolarization and activation of caspase-3 in
CHO cells were initiated within 2 hours of treatments. The onset of the apoptosis induction
was indistinguishable among the MDG, DFMDG and DFDG, and similar to that of
staurosporine.
Conclusion: The study shows that CHO cells treated with MDG, DFMDG and DFDG
undergo apoptotic death through the mitochondrial (intrinsic) pathway.
Description
Keywords
Apoptosis, Mitochondrial pathway, Glucopyranosides, Mitochondrial permeability transition pore
Citation
Lyantagaye, S.L., 2014. Characterization of the Biochemical Pathway of Apoptosis Induced by D-glucopyranoside Derivatives from Tulbaghia violacea. Annual Research & Review in Biology, 4(6), p.962.