Lignin-degrading Enzyme Production by Bjerkandera Adusta Immobilized on Polyurethane Foam

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dc.contributor.authorNakamura, Yoshitoshi
dc.contributor.authorMtui, Godliving Y. S.
dc.contributor.authorSawada, Tatsuro
dc.contributor.authorKuwahara, Masaaki
dc.date.accessioned2016-09-21T16:13:41Z
dc.date.available2016-09-21T16:13:41Z
dc.date.issued1999-10
dc.descriptionFull text can be accessed at http://www.sciencedirect.com/science/article/pii/S138917239980173Xen_US
dc.description.abstractProduction of the lignin-degrading enzymes lignin peroxidase (Lip), manganese peroxidase (MnP), and laccase (Lac) by the white-rot fungus Bjerkandera adusta was investigated experimentally using polyurethane foam (PUF) as a carrier of immobilized fungal mycelia. An immobilized cell culture with a low-nitrogen medium yielded significantly greater LiP, MnP, and Lac activities in comparison with those obtained in a liquid culture. The maximum activities of the three enzymes were 450, 370, and 100 U/ml, respectively, under the following incubation condition: glucose concentration, 20 g/l; temperature, 30°C; pH 4.5. The activities of MnP and Lac were significantly higher than those reported using other incubation methods. Lignin was degraded to the extent of 40% and its decolorization ratio was about 70% at an incubation time of 40 h using lignin-degrading enzymes from B. adusta. Six different isozymes of MnP were synthesized by B. adusta, two of which exhibited high MnP activity. Our preliminary finding that extracellular enzymes from B. adusta are capable of degrading and decoloring lignin makes these enzymes attractive for further research aimed at their large-scale application in lignin depolymerization, pulp biobleaching, and the degradation of toxic pollutants.en_US
dc.identifier.citationNakamura, Y., Sungusia, M.G., Sawada, T. and Kuwahara, M., 1999. Lignin-degrading enzyme production by Bjerkandera adusta immobilized on polyurethane foam. Journal of bioscience and bioengineering, 88(1), pp.41-47.en_US
dc.identifier.doi10.1016/S1389-1723(99)80173-X
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4050
dc.language.isoenen_US
dc.subjectBjerkandera adustaen_US
dc.subjectImmobilizationen_US
dc.subjectLignin peroxidaseen_US
dc.subjectManganese peroxidaseen_US
dc.subjectLaccaseen_US
dc.titleLignin-degrading Enzyme Production by Bjerkandera Adusta Immobilized on Polyurethane Foamen_US
dc.typeJournal Articleen_US
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