Isolation of the Tephrosia vogelii extract and rotenoids and their toxicity in the RTgill-W1 trout cell line and in zebrafish embryos

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dc.contributor.authorSaid, A.H.
dc.contributor.authorSolhaug, A.
dc.contributor.authorSandvik, M.
dc.contributor.authorMsuya, F.E.
dc.contributor.authorKyewalyanga, M.S.
dc.contributor.authorMmochi, A.J.
dc.contributor.authorLyche, J.L.
dc.contributor.authorHurem, S.
dc.date.accessioned2021-03-30T08:40:02Z
dc.date.available2021-03-30T08:40:02Z
dc.date.issued2020-05-23
dc.description.abstractThis study focused on identifying the rotenoids from the Tephrosia vogelli plant (fish-poison-bean), investigating the toxic potency of a crude T. vogelii extract and individual rotenoids (tephrosin, deguelin and rotenone) in vitro and in vivo and assessing the mode of action. A trout (Onychorynhis mykiss) gill epithelial cell line (RTgill-W1) was used to determine the cytotoxicity of rotenoids and effects on cell metabolism. Zebrafish (Danio rerio) aged from 3 h post fertilization (hpf) to 72 hpf were used for testing the developmental toxicity. The crude T. vogelii plant extract significantly decreased the cellular metabolic activity and was cytotoxic at lower concentrations (5 and 10 nM, respectively), while tephrosin, deguelin and rotenone showed these effects at concentrations � 50 nM. The crude T. Vogelli extract had the highest toxic potency and induced adverse health effects in zebrafish including deformities and mortality at the lowest concentration (5 nM) compared to rotenone (10 nM) and deguelin and tephrosin (50 nM). These results indicate that the crude T. Vogelii extracts are highly potent and the bioactivity of these extracts warrant further investigation for their potential use to treat parasites in human and veterinary medicine and as a natural alternative to pesticides.en_US
dc.description.sponsorshipsupported by the Norwegian Research Council funded through the Norwegian Agency for Development Cooperation (NORAD) and the Norwegian Programme for Capacity Building in Higher Education and Research for Development (NORHED) (project number Tan-13/0027). It was part of the TRAHESA (Capacity Building for Training and Research in Aquatic and Environmental Health in Eastern and Southern Africa) Project.en_US
dc.identifier.citationSaid, A.H., Solhaug, A., Sandvik, M., Msuya, F.E., Kyewalyanga, M.S., Mmochi, A.J., Lyche, J.L. and Hurem, S. (2020). Isolation of the Tephrosia vogelii extract and rotenoids and their toxicity in the RTgill-W1 trout cell line and in zebrafish embryos. Toxicon, 183: 51-60.en_US
dc.identifier.doihttps://doi.org/10.1016/j.toxicon.2020.05.013
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5566
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.subjectT. vogelii, Rotenoids, Cell-culture, Gill, Trout, Zebrafishen_US
dc.titleIsolation of the Tephrosia vogelii extract and rotenoids and their toxicity in the RTgill-W1 trout cell line and in zebrafish embryosen_US
dc.typeJournal Articleen_US
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