Optimized Production of Lignin Peroxidase, Manganese Peroxidase and Laccase in Submerged Cultures of Trametes Trogii Using Various Growth Media Compositions
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Date
2010
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Abstract
A white-rot fungus, Trametes trogii, was isolated from coastal Tanzania and screened for crude
lignolytic enzymes production using Rhemazol Brilliant blue R (RBBR) dye, 2,2-azino-bis (3-
ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Lignin peroxidase
(LiP), manganese peroxidase (MnP) and Laccase (Lac) were detected by pyrogallol and !-
napthol solutions, respectively on the guaiacol supplemented solid media. The effect of
temperature, pH, carbon, nitrogen, Cu2+, 2,5-xylidine, ferulic acid, varatryl alcohol and Mn2+ in
submerged culture fermentations were investigated for maximum enzymes production. After 7
days of incubation, 72-100% oxidation of RBBR, ABTS and guaiacol was observed. With
optimized culture conditions, the fungal filtrate had maximum LiP, MnP and Lac activities of
0.18, 4.44 and 593 U/ml, respectively compared to 0.0011, 0.0054 and 2.3 U/ml obtained with
non-optimized ones, amounting to 16,264%, 82,122% and 25,683% increase in LiP, MnP and
Lac activities, respectively. The enhanced crude enzymes activities, RBBR decolorization and
ABTS guaiacol oxidation capabilities of T. trogii show its potential as a source of industrial
enzymes for biotechnological applications.
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Keywords
Optimization, Trametes trogii, Lignin peroxidase, Manganese peroxidase, Laccase, Fermentation, Submerged
Citation
Patrick, F., Mtui, G., Mshandete, A.M. and Kivaisi, A., 2010. Optimized Production of Lignin Peroxidase, Manganese Peroxidase and Laccase in Submerged Cultures of Trametes Trogii Using Various Growth Media Compositions. Tanzania Journal of Science, 36(1).