Dietary magnesium improves growth, antioxidant capacity, ammonia tolerance and intestinal health of juvenile freshwater prawn, Macrobrachium nipponense

Simple item page
dc.contributor.authorYouqin Kong
dc.contributor.authorZhili Ding
dc.contributor.authorShanshan Wei
dc.contributor.authorChangle Qi
dc.contributor.authorYan Liu
dc.contributor.authorYixiang Zhang
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorJinyun Ye
dc.date.accessioned2022-08-05T17:51:58Z
dc.date.available2022-08-05T17:51:58Z
dc.date.issued2022-11-15
dc.description.abstractThe present study evaluated the effects of magnesium (Mg) on growth, antioxidant capacity, intestinal morphology and ammonia nitrogen resistance in juvenile oriental river prawn, Macrobrachium nipponense. Six semipurified diets (crude protein 401.1 g kg−1 diet; crude lipid 88.7 g kg−1 diet) containing Mg levels of 1.1, 1.4, 1.6, 2.1, 2.8 or 4.5 g kg−1 were formulated. A total of 1080 healthy juvenile prawns (0.151 ± 0.003 g) were randomly stocked into eighteen 300 L tanks (100 × 80 × 60 cm), each tank with 60 prawns. Each experimental diet was fed to prawns in triplicate twice daily (08:00 and 17:00) for eight weeks. After the feeding trial, 20 prawns from each tank were challenged with 37 mg/L ammonia nitrogen for 96 h, and the hemolymph, hepatopancreas and muscle of the rest of the prawns were collected. Final biomass was higher in prawns fed diets containing 1.6 and 2.1 g Mg kg−1 compared with those fed the diet with 1.1 g kg−1. Higher alkaline phosphatase (ALP) activity was obtained in the serum of prawns fed the 2.1 and 2.8 g Mg kg−1 diets. The highest activities of hepatopancreas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were observed in prawns fed 1.6–2.8 g Mg kg−1 of feed, and the lowest hepatopancreas malondialdehyde (MDA) concentrations and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were found in these treatment groups. Similarly, the prawns fed diets with 1.6 and 2.1 g Mg kg−1 had significantly lower MDA concentrations and cumulative mortality and higher antioxidant enzyme activities after ammonia nitrogen exposure. The intestinal epithelial cells were closely attached to the membrane in prawns fed the 2.1 g Mg kg−1 diet, but they were partly detached in those fed the 1.1 and 4.5 g Mg kg−1 diets. The minimum dietary Mg supplementations of juvenile M. nipponense were determined to be 1.6, 1.9, 2.0 and 2.2 g kg−1 feed, respectively, using broken-line regression analysis based on the final biomass, SOD, ALT and ALP.en_US
dc.description.sponsorshipZhejiang Province Public Welfare Technology Application Research Project (CN) (No. LGN21C190004, LGN21C190003)en_US
dc.identifier.citationYouqin Kong, Zhili Ding, Shanshan Wei, Changle Qi, Yan Liu, Yixiang Zhang, Samwel Mchele Limbu and Jinyun Ye (2022). Dietary magnesium improves growth, antioxidant capacity, ammonia tolerance and intestinal health of juvenile freshwater prawn, Macrobrachium nipponense, Aquaculture, 560; 738557 https://doi.org/10.1016/j.aquaculture.2022.738557en_US
dc.identifier.doihttps://doi.org/10.1016/j.aquaculture.2022.738557
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5883
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectMacrobrachium nipponenseen_US
dc.subjectMagnesium requirementen_US
dc.subjectAntioxidant statusen_US
dc.subjectGut histologyen_US
dc.subjectAmmonia nitrogenen_US
dc.titleDietary magnesium improves growth, antioxidant capacity, ammonia tolerance and intestinal health of juvenile freshwater prawn, Macrobrachium nipponenseen_US
dc.typeJournal Article, Peer Revieweden_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dietary magnesium improves the growth, antioxidant capacity, ammonia.pdf
Size:
1.03 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Department of Aquaculture Technology