Production of Raw Starch-Degrading Enzyme by Aspergillus Sp. And Its Use in Conversion of Inedible Wild Cassava Flour to Bioethanol

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dc.contributor.authorMoshi, Anselm P.
dc.contributor.authorHosea, Ken M.
dc.contributor.authorElisante, Emrode
dc.contributor.authorMamo, Gashaw
dc.contributor.authorÖnnby, Linda
dc.contributor.authorNges, Ivo A.
dc.date.accessioned2016-06-26T16:58:27Z
dc.date.available2016-06-26T16:58:27Z
dc.date.issued2016
dc.descriptionFull text can be accessed at http://www.sciencedirect.com/science/article/pii/S1389172315003321en_US
dc.description.abstractThe major bottlenecks in achieving competitive bioethanol fuel are the high cost of feedstock, energy and enzymes employed in pretreatment prior to fermentation. Lignocellulosic biomass has been proposed as an alternative feedstock, but because of its complexity, economic viability is yet to be realized. Therefore, research around non-conventional feedstocks and deployment of bioconversion approaches that downsize the cost of energy and enzymes is justified. In this study, a non-conventional feedstock, inedible wild cassava was used for bioethanol production. Bioconversion of raw starch from the wild cassava to bioethanol at low temperature was investigated using both a co-culture of Aspergillus sp. and Saccharomyces cerevisiae, and a monoculture of the later with enzyme preparation from the former. A newly isolated strain of Aspergillus sp. MZA-3 produced raw starch-degrading enzyme which displayed highest activity of 3.3 U/mL towards raw starch from wild cassava at 50°C, pH 5.5. A co-culture of MZA-3 and S. cerevisiae; and a monoculture of S. cerevisiae and MZA-3 enzyme (both supplemented with glucoamylase) resulted into bioethanol yield (percentage of the theoretical yield) of 91 and 95 at efficiency (percentage) of 84 and 96, respectively. Direct bioconversion of raw starch to bioethanol was achieved at 30°C through the co-culture approach. This could be attractive since it may significantly downsize energy expenses.en_US
dc.identifier.citationMoshi, A.P., Hosea, K.M., Elisante, E., Mamo, G., Önnby, L. and Nges, I.A., 2016. Production of raw starch-degrading enzyme by Aspergillus sp. and its use in conversion of inedible wild cassava flour to bioethanol. Journal of bioscience and bioengineering, 121(4), pp.457-463.en_US
dc.identifier.doi10.1016/j.jbiosc.2015.09.001
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2664
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectAspergillus sp.en_US
dc.subjectRaw starch degrading enzymeen_US
dc.subjectWild inedible cassavaen_US
dc.subjectBioethanolen_US
dc.subjectCo-cultureen_US
dc.subjectMonocultureen_US
dc.titleProduction of Raw Starch-Degrading Enzyme by Aspergillus Sp. And Its Use in Conversion of Inedible Wild Cassava Flour to Bioethanolen_US
dc.typeJournal Articleen_US
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