Effects of Temperature and Ph on the Growth of Heterotrophic Bacteria in Waste Stabilization Ponds. Water Research, 30, 447-455

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dc.contributor.authorMayo, Aloyce W.
dc.contributor.authorNoike, Tatsuya
dc.date.accessioned2016-05-25T08:38:18Z
dc.date.available2016-05-25T08:38:18Z
dc.date.issued1996
dc.descriptionFull text can be accessed at http://www.sciencedirect.com/science/article/pii/0043135495001506en_US
dc.description.abstractThis paper presents the effects of temperature and pH on the growth of heterotrophic bacteria in Chlorella vulgaris-heterotrophic bacteria culture. The growth of heterotrophic bacteria was studied at 10, 15, 20 and 30°C, and pH was controlled from 3.0 to 11.5 in a series of fed-batch chemostat reactors supplied with glucose as the sole source of carbon. Samples were analyzed for heterotrophic bacteria by tryptone glucose extract agar in triplicate. The agar pH of 7.0 was the best for enumeration of heterotrophic bacteria. The bacteria grown at pH near neutral were more sensitive to the variation of agar pH than those grown at very alkaline pH. No significant difference in the number of cells capable of forming colonies was noted for incubation temperature of 20 and 35°C, but the lag time for colony formation was longer at 20°C. Samples for enumeration of heterotrophic bacteria collected from algal-bacterial systems such as waste stabilization ponds are recommended to be incubated at 35°C for 72 h. Depending on the pH of the culture, about 86–98% of the cells capable of forming colonies will be visible to the naked eyes after incubation at 35°C for 72 h. At the steady state conditions, heterotrophic bacteria were not sensitive to temperature in the range of 10–20°C. However, at 30°C, a notable competition for glucose between Chlorella vulgaris and heterotrophic bacteria was observed. This competition was responsible for the low bacterial density near neutral pH. No evidence was found to support the view that the discharge of bactericidal substances from Chlorella vulgaris was responsible for reduction of heterotrophic bacteria at high pH.en_US
dc.identifier.citationMayo, A.W. and Noike, T., 1996. Effects of temperature and pH on the growth of heterotrophic bacteria in waste stabilization ponds. Water Research, 30(2), pp.447-455.en_US
dc.identifier.doi10.1016/0043-1354(95)00150-6
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2236
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectHeterotrophic bacteriaen_US
dc.subjectTemperatureen_US
dc.subjectpHen_US
dc.subjectAlgal-bacterial systemen_US
dc.subjectChlorella vulgarisen_US
dc.subjectWaste stabilization pondsen_US
dc.subjectCompetition for substrateen_US
dc.titleEffects of Temperature and Ph on the Growth of Heterotrophic Bacteria in Waste Stabilization Ponds. Water Research, 30, 447-455en_US
dc.typeJournal Articleen_US
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