Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins

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dc.contributor.authorMbuna, Julius
dc.contributor.authorKaneta, Takashi
dc.date.accessioned2016-07-14T20:46:52Z
dc.date.available2016-07-14T20:46:52Z
dc.date.issued2014-12
dc.description.abstractCapillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) is a powerful method for the trace analysis of cellular components. This review presents a summary of topics for the direct analysis of anthracyclines and multidrug resistance proteins in cancerous cells. A micellar electrokinetic chromatography (MEKC) method that does not use organic solvents, and hence prevents the precipitation of proteins in cellular samples, was shown to be a reliable method for the determination of several anthracyclines including the epimeric doxorubicin and epirubicin. A fast CE-based immunoassay for investigating transporter multidrug resistance associated protein (MRP1) was also developed for routine or explorative analysis of the levels of transporter proteins in cancerous cells. A combination of the developed MEKC-LIF method and the CE immunoassay (CEIA) method has permitted the analysis of anthracyclines and MRP1 in a cell line to show the relationship between the levels of MRP1 and amount of anthracyclines in cancerous cells.en_US
dc.identifier.citationMbuna, J. and Kaneta, T., 2015. Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins. Analytical Sciences, 31(11), pp.1121-1128.en_US
dc.identifier.doiDOI: 10.2116/analsci.31.1121
dc.identifier.urihttp://hdl.handle.net/20.500.11810/3189
dc.language.isoenen_US
dc.subjectCapillary electrophoresisen_US
dc.subjectMicellar electrokinetic chromatographyen_US
dc.subjectCapillary electrophoresis immunoassayen_US
dc.subjectLaser-induced fluorescenceen_US
dc.subjectAnthracyclineen_US
dc.subjectMultidrug resistance associated proteinen_US
dc.subjectCancer cellen_US
dc.titleCapillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteinsen_US
dc.typeJournal Article, Peer Revieweden_US
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