Identification of Mushroom Mycelia using DNA Techniques

Simple item page
dc.contributor.authorMuruke, M.H.S
dc.contributor.authorKivaisi, A.K
dc.contributor.authorMagingo, Francis S.
dc.contributor.authorDanell, E.
dc.date.accessioned2016-09-21T14:13:56Z
dc.date.available2016-09-21T14:13:56Z
dc.date.issued2002
dc.description.abstractThe suitability of using DNA techniques in the determination of relatedness of mushroom fruiting bodies to isolated mycelia was examined. Nine isolates of edible mushroom mycelia of general Oudemansiella, Coprinus and Pleurotus were identified using fruiting bodies as references. Polymerase Chain Reaction (PCR) in conbination with Restriction Fragment Length Polymorphism (RLFP) analyses were carried out on fruiting bodies and mycelia of the isolates. The internally transcribed spacer region (ITS) and ribosomal RNA gene (rDNA) was amplified using ITS1 and ITS4 primers. The RLFP analysis was carried out on the regions amplified by PCR from fruiting bodies and the mycelia was established by looking at DNA fragment band sizes and patterns. Banding patterns and fragment sizes of DNA obtained from mycelia and their corresponding fruiting bodies were identical and characteristic for the species. Using this technique, it was possible to sort out a case of mistaken identity of Oudemansiella fruiting bodies, which were interchanged with another mushroom specimen during packing. The method is fast, accurate, and could be used for routine screening of edible mushrooms of Tanzania for taxonomical purposes. For the latter purpose, it is required that the RFLP database of taxonomically known species is in place.en_US
dc.identifier.citationMuruke, M.H.S., Kivaisi, A.K., Magingo, F.S.S. and Danell, E., 2002. Identification of mushroom mycelia using DNA techniques. Tanzania Journal of Science, 28(1), pp.115-128.en_US
dc.identifier.doi.org/10.4314/tjs.v28i1.18323
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4015
dc.language.isoenen_US
dc.titleIdentification of Mushroom Mycelia using DNA Techniquesen_US
dc.typeJournal Article, Peer Revieweden_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Identification of mushroom mycelia using DNA techniques.pdf
Size:
19.74 MB
Format:
Adobe Portable Document Format
Description:
Full text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Department of Molecular Biology and Biotechnology