High carbohydrate diet partially protects Nile tilapia from oxytetracycline-induced side effects. Environmental Pollution, 256: 113508.

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dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorHan Zhang
dc.contributor.authorYuan Luo
dc.contributor.authorMeiling Zhang
dc.contributor.authorZhen-Yu Du
dc.date.accessioned2021-04-18T05:51:10Z
dc.date.available2021-04-18T05:51:10Z
dc.date.issued2020-01
dc.description.abstractAntibiotics used in global aquaculture production cause various side effects, which impair fish health. However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear. We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects. Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days. Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis. To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets. The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively. Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet. The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet. Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet. The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively. Our study demonstrates that, high carbohydrate partially protects O. niloticus from oxytetracycline-induced side effects by binding the antibiotic. Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.en_US
dc.description.sponsorshipthe National Key Research and Development Program of China (2018YFD0900400), the Fundamental Research Funds for the Central Universities, and the Chinese Scholarship Council (CSC)en_US
dc.identifier.citationSamwel M. Limbu, Han Zhang, Yuan Luo, Meiling Zhang and Zhen-Yu Du (2020). High carbohydrate diet partially protects Nile tilapia from oxytetracycline-induced side effects. Environmental Pollution, 256: 113508. https://doi.org/10.1016/j.envpol.2019.113508en_US
dc.identifier.doihttps://doi.org/10.1016/j.envpol.2019.113508
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5609
dc.publisherElsevieren_US
dc.subjectOxytetracycline; Binding effect; Oreochromis niloticus; Apoptosis; Cellular stress; Hepatotoxicityen_US
dc.titleHigh carbohydrate diet partially protects Nile tilapia from oxytetracycline-induced side effects. Environmental Pollution, 256: 113508.en_US
dc.typeJournal Article, Peer Revieweden_US
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