Antimicrobial and Cytotoxicity Activity of Clausena Anisata, Acokanthera Shemperii and Olea Europaea Growing in Tanzania
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Date
2016
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Abstract
Aims: To evaluate antimicrobial and cytotoxicity activities of Clausena anisata, Acokanthera
shemperii and Olea europaea against seven Gram negative bacteria and fungal species.
Study Design: Bioassay of antimicrobial assay was done using 96-well micro-dilution method.
Place and Duration of Study: School of Life Science and Bioengineering, Nelson Mandela African
Institution of Science and Technology, Arusha, Tanzania, from April 2014 to June 2014.
Methodology: 96-well micro dilution method was used in antimicrobial assay. Extracts were
loaded in the wells of the first row, followed by serial dilution and 50 µl of the bacterial suspensions
(0.5 MacFarland standard turbidity) were added in each well. The first concentration which showed
no bacterial growth was considered as minimum inhibition concentration. Method developed by
Meyer et al 1982 was adopted in cytotoxicity activities.
Results: All extracts indicated antibacterial activity on at least three to five of the tested seven
bacteria and two fungi species with MIC value ranging 0.7812 - 12.5 mg/mL. The highest activity was demonstrated by Olea europaea leaf methanolic, Acokanthera shemperii stem bark and
Clausena anisata twigs ethyl acetate extracts with MIC value of 0.7812 mg/mL against
Pseudomonas aeruginosa while the same MIC value was exhibited by Olea europaea stem bark
methanol against Proteus mirabilis. However the Olea europaea root methanolic extract inhibited
the growth of Pseudomonas aeruginosa and Salmonella kisarawe with MIC value of 0.7812 mg/mL.
Olea europaea leaf methanolic and stem bark methanolic which demonstrated high antimicrobial
activity were non toxic against brine shrimp larvae with LC50 value of 369.8272 and 226.1566
µg/mL, while Clausena anisata twigs ethyl acetate, Acokanthera shemperii stem bark ethyl acetate
and Olea europaea root methanolic extracts were toxic with LC50 value of 6.21276, 67.4179 and
92.3089 µg/mL respectively.
Conclusion: This study has unveiled antimicrobial and cytotoxicity properties of Clausena anisata,
Acokanthera shemperii and Olea europaea.
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Keywords
Antimicrobial, Clausena anisata, Acokanthera shemperii, Olea europaea
Citation
Makirita, W.E., Chauka, L.J. and Chacha, M., 2016. Antimicrobial and Cytotoxicity Activity of Clausena anisata, Acokanthera shemperii and Olea europaea Growing in Tanzania. European Journal of Medicinal Plants, 14(2).