In Planta - Complementation of Clavibacter Michiganensis Subsp. sepedonicus Strains Deficient in Cellulase Production or HR Induction Restores Virulence

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dc.contributor.authorNissinen, Riitta
dc.contributor.authorKassuwi, shaaban A. A.
dc.contributor.authorPeltola, Riikka
dc.contributor.authorMetzler, Mary C.
dc.date.accessioned2016-07-21T18:22:13Z
dc.date.available2016-07-21T18:22:13Z
dc.date.issued2001-01
dc.descriptionFull text can be accessed at http://link.springer.com/article/10.1023/A:1011270926064en_US
dc.description.abstractClavibacter michiganensis subsp. sepedonicus, a Gram positive bacterium that causes bacterial ring rot of potato, was studied in eggplant, an alternate host, using strains that differed in phenotype. Two factors affecting virulence, the ability to induce a hypersensitive response (HR) and cellulase production, were studied. A plasmid-free isolate of C. michiganensis subsp. sepedonicus that causes HR on tobacco but is unable to produce cellulase multiplied efficiently in planta, but caused only weak symptoms. In contrast, a strain that is unable to induce HR on tobacco but produces cellulase was impaired in the ability to multiply in the host and caused no symptoms. When the two non-virulent strains were coinoculated into eggplants, typical disease symptoms developed. This enhancement was not due to formation of a new phenotype or significant increases in population density of either of the strains. Our results suggest that both cellulase production and the ability to induce HR are required for a successful infection process and disease induction by C. michiganensis subsp. sepedonicus. Our results additionally suggest that the ability to induce HR on non-host plants is required for multiplication in the host plant, whereas cellulase expression is necessary for induction of disease symptoms.en_US
dc.identifier.citationNissinen, R., Kassuwi, S., Peltola, R. and Metzler, M.C., 2001. In planta-complementation of Clavibacter michiganensis subsp. sepedonicus strains deficient in cellulase production or HR induction restores virulence. European journal of plant pathology, 107(2), pp.175-182.en_US
dc.identifier.doi10.1023/A:1011270926064
dc.identifier.urihttp://hdl.handle.net/20.500.11810/3368
dc.language.isoenen_US
dc.titleIn Planta - Complementation of Clavibacter Michiganensis Subsp. sepedonicus Strains Deficient in Cellulase Production or HR Induction Restores Virulenceen_US
dc.typeJournal Articleen_US
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