Fasting enhances cold resistance in fish through stimulation of lipid catabolism and autophagy

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dc.contributor.authorLu, Dong-Liang
dc.contributor.authorMa, Qiang
dc.contributor.authorNing, Li-Jun
dc.contributor.authorWang, Jing
dc.contributor.authorLi, Ling-Yu
dc.contributor.authorLi, Jia-Min
dc.contributor.authorHan, Si-Lan
dc.contributor.authorSun, Sheng-Xiang
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorLi, Dong-Liang
dc.contributor.authorQiao, Fang
dc.contributor.authorChen, Li-Qiao
dc.contributor.authorZhang, Mei-Ling
dc.contributor.authorDu, Zhen-Yu
dc.date.accessioned2019-05-08T05:49:31Z
dc.date.available2019-05-08T05:49:31Z
dc.date.issued2019-01-07
dc.description.abstractIn cold environments, most homeothermic animals increase their food intake to supply more energy to maintain body temperature, whereas most poikilothermic animals such as fishes decrease or even stop feeding under cold stress. However, the physiological value of fasting during cold resistance in poikilotherms has not been explained. Here, we show that moderate fasting largely enhanced cold resistance in fish. By using pharmacological (fenofibrate, mildronate, chloroquine and rapamycin) and nutritional approaches (fatty acids diets and amino acids diets) in wild‐type or specific gene knock‐out zebrafish models (carnitine palmitoyltransferase‐1b‐deficient strain, CPT1b−/−, or autophagy‐related protein 12‐deficient strain, ATG12−/−), we verified that fasting‐stimulated lipid catabolism and autophagy played essential roles in the improved cold resistance. Moreover, suppression of the mechanistic target of rapamycin (mTOR) pathway by using rapamycin mostly mimicked the beneficial effects of fasting in promoting cold resistance as either the physiological phenotype or transcriptomic pattern. However, these beneficial effects were largely reduced when the mTOR pathway was activated through high dietary leucine supplementation. We conclude that fasting helps fish to resist cold stress by modulating lipid catabolism and autophagy, which correlates with the mTOR signalling pathway. Therefore, fasting can act as a protective strategy of fish in resisting coldness.en_US
dc.description.sponsorshipNational Natural Science Fund (31830102, 31772859 and 31472290).en_US
dc.identifier.doihttps://doi.org/10.1113/JP277091
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5241
dc.publisherWileyen_US
dc.subjectCold resistanceen_US
dc.subjectFastingen_US
dc.subjectLipid catabolismen_US
dc.subjectAutophagyen_US
dc.subjectmTORen_US
dc.subjectFishen_US
dc.titleFasting enhances cold resistance in fish through stimulation of lipid catabolism and autophagyen_US
dc.typeJournal Article, Peer Revieweden_US
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