The presence or absence of intestinal microbiota affects lipid deposition and related genes expression in zebrafish (Danio rerio).

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dc.contributor.authorYi, Sheng
dc.contributor.authorHui, Ren
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorYuhong, Sun
dc.contributor.authorFang, Qiao
dc.contributor.authorWanying, Zhai
dc.contributor.authorZhen-Yu, Du
dc.contributor.authorMeiling, Zhang
dc.date.accessioned2019-05-07T14:21:43Z
dc.date.available2019-05-07T14:21:43Z
dc.date.issued2018-05-29
dc.description.abstractUnderstanding how intestinal microbiota alters energy homeostasis and lipid metabolism is a critical process in energy balance and health. However, the exact role of intestinal microbiota in the regulation of lipid metabolism in fish remains unclear. Here, we used two zebrafish models (germ-free and antibiotics-treated zebrafish) to identify the role of intestinal microbiota in lipid metabolism. Conventional and germ-free zebrafish larvae were fed with egg yolk. Transmission electron microscopy was used to detect the presence of lipid droplets in the intestinal epithelium. The results showed that, microbiota increased lipid accumulation in the intestinal epithelium. The mRNA sequencing technology was used to assess genes expression level. We found majority of the differentially expressed genes were related to lipid metabolism. Due to the limitation of germ-free zebrafish larvae, antibiotics-treated zebrafish were also used to identify the relationship between the gut microbiota and the host lipid metabolism. Oil-red staining showed antibiotics-treated zebrafish had less intestinal lipid accumulation than control group. The mRNA expression of genes related to lipid metabolism in liver and intestine was also quantified by using real-time PCR. The results indicated that apoa4, hsl, cox15, slc2a1a, and lss were more related to intestinal bacteria in fish, while the influence of intestinal microbiota on the activity of fabp6, acsl5, cd36, and gpat2 was different between the liver and intestine. This study identified several genes regulated by intestinal microbiota. Furthermore, the advantages and disadvantages of each model have been discussed. This study provides valuable information for exploring host-microbiota interactions in zebrafish in future.en_US
dc.description.sponsorshipThe National Basic Research Program (973 Program) (Grant No. 2014CB138603), the National Natural Science Foundation of China (Grant No. 31672668), the Science and Technology Commission of Shanghai Municipality (Grant No. 16ZR1409900), and the Open Funding Project of Key Laboratory of Exploration and Utilization of Aquatic Genetic Resourcesen_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2018.01124
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5225
dc.publisherFrontiers Media SAen_US
dc.subjectgerm-freeen_US
dc.subjectlipid metabolismen_US
dc.subjectzebrafishen_US
dc.subjectgut microbiotaen_US
dc.subjectantibioticen_US
dc.titleThe presence or absence of intestinal microbiota affects lipid deposition and related genes expression in zebrafish (Danio rerio).en_US
dc.typeJournal Article, Peer Revieweden_US
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