Antimicrobial and cytotoxicity Activity of Clausena anisata, Acokanthera shemperii and Olea europaea growing in Tanzania

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dc.contributor.authorMakirita W, Makirita W Chauka L and Chacha M
dc.date.accessioned2016-05-30T12:49:40Z
dc.date.available2016-05-30T12:49:40Z
dc.date.issued2016-04
dc.description.abstractAims: To evaluate antimicrobial and cytotoxicity activities of Clausena anisata, Acokanthera shemperii and Olea europaea against seven Gram negative bacteria and fungal species. Study Design: Bioassay of antimicrobial assay was done using 96-well micro-dilution method. Place and Duration of Study: School of Life Science and Bioengineering, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania, from April 2014 to June 2014. Methodology: 96-well micro dilution method was used in antimicrobial assay. Extracts were loaded in the wells of the first row, followed by serial dilution and 50 μl of the bacterial suspensions (0.5 MacFarland standard turbidity) were added in each well. The first concentration which showed no bacterial growth was considered as minimum inhibition concentration. Method developed by Meyer et al 1982 was adopted in cytotoxicity activities. Results: All extracts indicated antibacterial activity on at least three to five of the tested seven bacteria and two fungi species with MIC value ranging 0.7812 - 12.5 mg/mL. The highest activityen_US
dc.identifier.citationiv. Makirita W, Chauka L and Chacha M. (2016).Antimicrobial and cytotoxicity Activity of Clausena anisata, Acokanthera shemperii and Olea europaea growing in Tanzania. European Journal of Medicinal Plants, 14(2): 1-9en_US
dc.identifier.issn2231-0894
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2316
dc.publisherEuropean Journal of Medicinal Plantsen_US
dc.subjectMedicinal products from plantsen_US
dc.titleAntimicrobial and cytotoxicity Activity of Clausena anisata, Acokanthera shemperii and Olea europaea growing in Tanzaniaen_US
dc.typeJournal Articleen_US
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