Induction of Early Apoptosis and Reactive Oxygen Species (ROS) Production by Tanzanian Basidiomycete ( Cantharellus Miomboensis )

Simple item page
dc.contributor.authorMasalu, Rose
dc.contributor.authorHosea, Ken M.
dc.contributor.authorMeyer, Mervin
dc.contributor.authorLyantagaye, Sylvester L.
dc.contributor.authorKanyande, Stonard
dc.date.accessioned2016-06-26T17:30:48Z
dc.date.available2016-06-26T17:30:48Z
dc.date.issued2010
dc.description.abstractCantharellus miomboensis is a new basidiomycete fungus recently found in Miombo woodlands in Tanzania. In this study, crude extract was prepared from fruiting bodies of C. miomboensis and was in vitro screened for its cytotoxicity using Tetrazolium salt (3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) in human cell lines namely; Hepatocellular carcinoma (HepG2), Human non-small cell lung carcinoma (H157) and Human colon adenocarcinoma (HT.29). Thereafter, pro-apoptotic effects were determined using biochemical changes in apoptotic cells. These included externalization of phospholipid phosphatidylserine (PS) using APO Percentage dye by flow cytometry and depolarization of mitochondrial membrane potential using Tetramethyl rhodamine ethyl ester perchlorate (TMRE) assay. The test extract was found to induce dose dependent PS externalization on human cell lines when treated with various concentrations (1 - 5 mg/ml) and completely depolarized the mitochondrial membrane potential after 6 hours on HepG2 cell line. When the extract was examined for ROS production using 2’,7’- dichlorofluorescin diacetate (DCFH-DA) staining, there was no ROS generation found in HepG2 cells. It is therefore concluded that C. miomboensis extract is able to induce apoptosis in HepG2 cells and PS externalization and loss of mitochondrial membrane potential in HepG2 cells appear to be independent of ROS production.en_US
dc.identifier.citationMasalu, R., Hosea, K.M., Meyer, M., Lyantagay, S. and Kanyande, S., 2010. Induction of early apoptosis and reactive oxygen species (ROS) production by Tanzanian basidiomycete (Cantharellus miomboensis). International Journal of Biological and Chemical Sciences, 4(4).en_US
dc.identifier.doi10.4314/ijbcs.v4i4.62955
dc.identifier.issn1991-8631
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2759
dc.language.isoenen_US
dc.subjectMiombo woodlandsen_US
dc.subjectApoptosisen_US
dc.subjectBasidiomyceteen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectCarcinomaen_US
dc.titleInduction of Early Apoptosis and Reactive Oxygen Species (ROS) Production by Tanzanian Basidiomycete ( Cantharellus Miomboensis )en_US
dc.typeJournal Article, Peer Revieweden_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Induction of early apoptosis and reactive oxygen species (ROS) production by Tanzanian basidiomycete ( Cantharellus miomboensis ).pdf
Size:
330.49 KB
Format:
Adobe Portable Document Format
Description:
Full text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Department of Molecular Biology and Biotechnology