Genetic Characterization of Osmotolerant Fermentative Saccharomyces Yeasts from Tanzania Suitable for Industrial Very High Gravity Fermentation

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dc.contributor.authorSumari, Deborah
dc.contributor.authorHosea, Ken M.
dc.contributor.authorMagingo, F. S. S.
dc.date.accessioned2016-06-26T17:34:56Z
dc.date.available2016-06-26T17:34:56Z
dc.date.issued2010
dc.description.abstractNaturally occurring yeasts were sought from diverse Tanzanian environments and screened for industrial application. The yeasts were isolated from environments such as traditional brews and wines from various parts of Tanzania. In this regard, a total of thirty yeast isolates were screened for their suitability in Industrial Very High Gravity Fermentation (VHGF). Five of these isolates were able to grow and ferment medium with 40% initial sucrose concentration. Whereby, three were able to grow and ferment medium with 700 g/Litre (70% w/w) initial sucrose concentration. One of the three isolates coded LB2 isolated from a traditional Makonde sorghum brew was able to ferment a medium with initial sucrose concentration of 1000 g/Litre (100% w/w) at 30°C. On the basis of PCR-RFLP of the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA), all the three most osmotolerant isolates were identified to belong to the Saccharomyces sensu stricto clade. Phylogenetic analysis of the 650 bp D1D2 domain of the large subunit 26S rDNA of the isolate LB2 clustered this isolate away from the so far known typical osmotolerant yeasts. The fermentation by LB2 isolate of 100% gravity substrate observed in this work is higher than any other encountered in the literatures reviewed.en_US
dc.identifier.citationSumari, D., Hosea, K.M.M. and Magingo, F.S.S., 2010. Genetic characterization of osmotolerant fermentative Saccharomyces yeasts from Tanzania suitable for industrial very high gravity fermentation. African Journal of Microbiology Research, 4(11), pp.1064-1070.en_US
dc.identifier.issn1996-0808
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2762
dc.language.isoenen_US
dc.subjectOsmotoleranten_US
dc.subjectPCR-RFLPen_US
dc.subjectSaccharomycesen_US
dc.subjectTraditional brewsen_US
dc.subjectVery High Gravity Fermentation (VHGF)en_US
dc.titleGenetic Characterization of Osmotolerant Fermentative Saccharomyces Yeasts from Tanzania Suitable for Industrial Very High Gravity Fermentationen_US
dc.typeJournal Article, Peer Revieweden_US
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