Development of optimal Culture Method of Sparassis crispa mycelia and new extraction method of Antineoplastic Constituent.

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dc.contributor.authorKurosumi, Akihiro
dc.contributor.authorKobayasi, Fumihisa
dc.contributor.authorMtui, Godliving Y. S.
dc.contributor.authorNakamura, Yoshitoshi
dc.date.accessioned2016-09-21T14:21:45Z
dc.date.available2016-09-21T14:21:45Z
dc.date.issued2006-05
dc.description.abstractThe optimal culture condition of basidiomycete Sparassis crispa mycelia was determined for producing a large amount of antineoplastic constituent, i.e. β-1,3-glucan. Furthermore, a new extraction method with steam explosion as a pretreatment was attempted to increase the amount of antineoplastic constituent extracted from S. crispa mycelia. The shake-flask liquid culture of fungal mycelia could shorten a culture time up to about 1/3 compared with the conventional stationary liquid culture. The productivity of S. crispa mycelia reached its maximum at an initial glucose concentration of 30 g/L, a culture temperature of 25–30 °C, and pH 5. Steam explosion at a steam temperature of 225 °C for 5 min not only enhanced the amount of antineoplastic constituent extracted from the mycelia but also shortened the extraction time significantly.en_US
dc.identifier.citationKurosumi, A., Kobayasi, F., Mtui, G. and Nakamura, Y., 2006. Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochemical engineering journal, 30(1), pp.109-113.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4030
dc.language.isoenen_US
dc.subjectBioconversionen_US
dc.subjectFilamentous fungien_US
dc.subjectβ-1,3-Glucanen_US
dc.subjectMicrobial growthen_US
dc.subjectPlant cell cultureen_US
dc.subjectSparassis crispaen_US
dc.titleDevelopment of optimal Culture Method of Sparassis crispa mycelia and new extraction method of Antineoplastic Constituent.en_US
dc.typeJournal Article, Peer Revieweden_US
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