In Vitro Antiplasmodial and HIV-1 Neutralization Activities of Root and Leaf Extracts From Berberis Holstii

Simple item page
dc.contributor.authorNgwira, Kennedy
dc.contributor.authorMaharaj, Vinesh
dc.contributor.authorMgani, Q.A.
dc.date.accessioned2016-06-26T17:13:52Z
dc.date.available2016-06-26T17:13:52Z
dc.date.issued2015-01
dc.description.abstractThe in vitro antiplasmodial activities and HIV neutralizing activities of six crude extracts from the leaves and roots of Berberis holstii were evaluated. The antiplasmodial activities were assessed against a chloroquine sensitive 3D7 strain of the malaria parasite, Plasmodium falciparum, and the HIV neutralizing activity were assessed against HBX2 and ZM53 pseudovirions. Parasite Lactate Dehydrogenase (pLDH) assay was used to measure parasite viability and Luciferase Reporter Gene assay was used to measure HIV neutralizing activity. The crude alkaloid extract of the roots showed the most potent anti-parasitic activity (IC50 = 0.17 μg/mL) with a selectivity index (SI) of 701.2 while the DCM/MeOH extract also showed good activity (IC50 = 2.33 μg/mL). The aqueous and total alkaloid extracts of the roots displayed significant anti-parasitic activity (IC50 = 3.64 and 3.85 μg/mL respectively). The aqueous extract of the leaves displayed good HIV neutralizing activity (IC50 = 5.84 μg/mL) against the HXB2 pseudo virus. All the extracts tested displayed low cytotoxicity against TZM-bl cells.en_US
dc.identifier.citationNgwira, K.J., Maharaj, V.J. and Mgani, Q.A., 2015. In vitro antiplasmodial and HIV-1 neutralization activities of root and leaf extracts from Berberis holstii. Journal of Herbal Medicine, 5(1), pp.30-35.en_US
dc.identifier.doi10.1016/j.hermed.2014.12.001
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2720
dc.language.isoenen_US
dc.subjectBerberis holstiien_US
dc.subjectAntiplasmodial activityen_US
dc.subjectHIV neutralizationen_US
dc.subjectCytotoxicityen_US
dc.titleIn Vitro Antiplasmodial and HIV-1 Neutralization Activities of Root and Leaf Extracts From Berberis Holstiien_US
dc.typeJournal Article, Peer Revieweden_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Beberines publication Kennedy.pdf
Size:
1.07 MB
Format:
Adobe Portable Document Format
Description:
Full text
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Chemistry Department