Rapid Purification of Uricase from Bacillus Fastidiosus
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Abstract
A simple, rapid procedure was developed for the purification of uricase from Bacillus fastidiosus. The enzyme was purified to homogeneity in a two-step procedure with the aid of fast-flow column chromatography. In this way high yields (37 %) of pure uricase with a specific activity of 78.3 U/mg were obtained in a short time.
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Full text can be accessed at
http://link.springer.com/article/10.1007/BF00168485
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Muruke, M.S.H., den Camp, H.O. and Van der Drift, C., 1996. Rapid purification of uricase from Bacillus fastidiosus. Biotechnology techniques, 10(9), pp.709-712.