Combined Thermo-Microbial Pre-Treatments Methods for Enhanced Biogas Production from Nile Perch Fish Solid Waste in Tanzania

Abstract
Anaerobic digestion (AD) of fish waste is difficult mainly due to high protein and lipid content which inhibit the process. Pre-treatments methods are option to improved AD of fish wastes for biogas production. Effects of heating, pasteurization separately and combined with CBR-11 bacteria strain culture (internal code) pre-treatment on biogas production from fish solid waste (FSW) was investigated in batch anaerobic bioreactors (BAB). The two thermo pre-treatment methods had a positive effect with best results 311 and 361 CH 4 ml/g volatile solids (VS) added obtained from heated FSW for 90 seconds at 130 o C and pasteurised FSW for 60 minutes at 70 o C, respectively. The highest methane yield were two fold compared with non-pre-treated FSW (163 CH 4 ml/gVS added). Heated and pasteurised FSW each separately combined with CBR 11 bacterial culture pre-treatment prior to AD had a maximum methane yield of 697 and 491 CH 4 ml/gVS added , respectively. Resulting in three to four fold increases in methane yield compared with non-pre-treated FSW (163 CH 4 ml/gVS added). The methane content of the biogas ranged between 70-81% regardless of pre-treatment methods employed. In conclusion combined thermo-microbial pre-treatment is a novel method for enhanced biogas production of FSW. However, remain to be tested in continuous anaerobic digester with or without co-digestion.
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