Cytosolic Enzymes with a Mitochondrial Ancestry from the Anaerobic Chytrid Piromyces Sp. E2. Mol Microbiol 30:1017-1027

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dc.contributor.authorAkhmanova, Anna
dc.contributor.authorVoncken, Frank
dc.contributor.authorHarhangi, Harry R.
dc.contributor.authorHosea, Ken M.
dc.contributor.authorVogels, Godfried D.
dc.contributor.authorHackstein, Johannes H. P.
dc.date.accessioned2016-06-26T17:11:00Z
dc.date.available2016-06-26T17:11:00Z
dc.date.issued1998
dc.description.abstractThe anaerobic chytrid Piromyces sp. E2 lacks mitochondria, but contains hydrogen-producing organelles, the hydrogenosomes. We are interested in how the adaptation to anaerobiosis influenced enzyme compartmentalization in this organism. Random sequencing of a cDNA library from Piromyces sp. E2 resulted in the isolation of cDNAs encoding malate dehydrogenase, aconitase and acetohydroxyacid reductoisomerase. Phylogenetic analysis of the deduced amino acid sequences revealed that they are closely related to their mitochondrial homologues from aerobic eukaryotes. However, the deduced sequences lack N-terminal extensions, which function as mitochondrial leader sequences in the corresponding mitochondrial enzymes from aerobic eukaryotes. Subcellular fractionation and enzyme assays confirmed that the corresponding enzymes are located in the cytosol. As anaerobic chytrids evolved from aerobic, mitochondria-bearing ancestors, we suggest that, in the course of the adaptation from an aerobic to an anaerobic lifestyle, mitochondrial enzymes were retargeted to the cytosol with the concomitant loss of their N-terminal leader sequences.en_US
dc.identifier.citationAkhmanova, A., Voncken, F.G., Harhangi, H., Hosea, K.M., Vogels, G.D. and Hackstein, J.H., 1998. Cytosolic enzymes with a mitochondrial ancestry from the anaerobic chytrid Piromyces sp. E2. Molecular microbiology, 30(5), pp.1017-1027.en_US
dc.identifier.doi10.1046/j.1365-2958.1998.01130.x
dc.identifier.urihttp://hdl.handle.net/20.500.11810/2689
dc.language.isoenen_US
dc.titleCytosolic Enzymes with a Mitochondrial Ancestry from the Anaerobic Chytrid Piromyces Sp. E2. Mol Microbiol 30:1017-1027en_US
dc.typeJournal Article, Peer Revieweden_US
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