Department of Molecular Biology and Biotechnology

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Browsing Department of Molecular Biology and Biotechnology by Subject "AFLP"

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    AFLP Markers Support Separation of Solanum Nodiflorum from Solanum Americanum Sensu Stricto (Solanaceae)
    (Springer Link, 2007) Manoko, Mkabwa L. K.; van den Berg, Ronald G.; Feron, Richard M. C.; van der Weerden, Gerard M.; Mariani, Celestina
    This study was aimed at examining the relationships between the African material of Solanum americanum (also designated as S. nodiflorum), accessions of this taxon from other geographical areas, and American S. americanum using AFLP markers. 96 individuals representing 39 accessions of S. americanum sensu lato and related diploid species from the widest possible geographical range, and one accession of S. dulcamara (as outgroup) were used. The AFLP results suggested that American S. americanum differs from S. nodiflorum and that the material investigated in this study can be assigned to three different species: S. americanum sensu stricto, S. nodiflorum and a Solanum species from Brazil. These species can be differentiated based on a combination of floral and fruit characteristics.
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    Genetic Diversity of the African hexaploid Species Solanum scabrum Mill. and Solanum nigrum L. (Solanaceae)
    (2008) Manoko, Mkabwa L. K.; van den Berg, Ronald G.; Feron, Richard M. C.; van der Weerden, Gerard M.; Mariani, Celestina
    Two hexaploid species of Solanum sect. Solanum are present in Africa: Solanum scabrum and S. nigrum. Solanum scabrum is a widely cultivated species and is used as a leafy vegetable, as a source of medicine and as a source of ink dye. In previous studies a wide range of morphological diversity has been reported in this species and in some studies subspecies have been proposed. Subspecies are also recognized in S. nigrum. However, it has not been established whether or not the morphological differences are reflected at the genomic level. The present study applies AFLPs to study the genetic diversity in S. scabrum and its relationship to geographical provenance, morphological differences and the possible existence of subspecies within S. scabrum and S. nigrum. The data obtained were analyzed with cluster analysis (using UPGMA and NJ). The results indicate that the genetic variation within S. scabrum was higher within accessions than between accessions. Accessions did not cluster according to their geographical provenance, indicating that accessions from different geographical areas were not significantly different genetically. The clustering reflected neither morphological differences nor domestication status (cultivated or wild). The morphological differences exhibited by S. scabrum could be due to selection by farmers for different plant types. The AFLP derived clustering pattern did not segregate the subspecies recognized in S. scabrum and S. nigrum into separate subclusters.