Browsing by Author "Shpigelman, Avi"

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    Pectin modifications and the Role of Pectin-degrading Enzymes during Postharvest Softening of Jonagold Apples
    (Applied Science Publishers, 2014) Gwanpua, Sunny George; Van Buggenhout, Sandy; Verlinden, Bert; Christiaens, Stefanie; Shpigelman, Avi; Vicent, Victor; Jamsazzadeh Kermani, Zahra; Nicolaï, Bart; Hendrickx, Marc; Geeraerd, Annemie
    This study aimed at understanding softening in Jonagold apple (Malus × domestica Borkh.) fruits, by investigating pectin modifications and the evolution of pectin-modifying enzymes during postharvest storage and ripening. Jonagold apples were harvested at commercial maturity and stored at different temperatures and controlled atmosphere conditions for 6 months, followed by exposure to ambient shelf life conditions (20 °C under air) for 2 weeks. The composition of the pectic material was analysed. Furthermore, the firmness and the ethylene production of the apples were assessed. Generally, the main changes in pectin composition associated with the loss of firmness during ripening in Jonagold apples were a loss of side chains neutral sugars, increased water solubility and decreased molar mass. Also, the activities of four important enzymes possibly involved in apple softening, β-galactosidase, α-arabinofuranosidase, polygalacturonase and pectin methylesterase, were measured. Pectin-related enzyme activities highly correlated with ethylene production, but not always with pectin modifications.