Browsing by Author "Shanshan Wei"

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    Adverse effects of chronic ammonia stress on juvenile oriental river prawn (Macrobrachium nipponense) and alteration of glucose and ammonia metabolism
    (Wiley, 2023-03) Shanshan Wei; Jin Zhang; Wanxin Chen; Anfu Shen; Dongsheng Zhou; Jinxian Zheng; Habib Thiam; Zhili Ding; Samwel Mchele Limbu; Youqin Kong
    Ammonia is one of the common stress factors in aquaculture. However, the effect of chronic ammonia exposure in juvenile oriental river prawn (Macrobrachium nipponense) is currently unexplored. This study explored the effects of chronic ammonia on juvenile healthy oriental river prawns. Fifty prawns (0.123 ± 0.003 g) were exposed to 0, 5, and 15 mg/L total ammonia nitrogen (TAN) in triplicates for 28 days. The effects of chronic ammonia challenge were evaluated on growth, antioxidant capacity, hepatopancreas and gill morphology, and glucose and ammonia metabolism. The results showed that, the chronic ammonia exposure reduced significantly survival rate and weight gain of prawns. The prawns exposed to 15 mg/L ammonia had induced oxidative stress. However, the prawn exposed to 15 mg/L ammonia had significantly lower aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and acid phosphatase activities in the serum. Furthermore, exposure of prawns to 15 mg/L ammonia increased the activities of hexokinase, pyruvate kinase, pyruvate and lactic acid content, and glutamine synthase activity. However, the prawns exposed to 15 mg/L ammonia, reduced succinic dehydrogenase, 6-phosphogluconic dehydrogenase, phosphoenolpyruvate carboxykinase, glutamate synthase, and glutamate dehydrogenase activities but increased ammonia content in serum. The exposure of ammonia deformed lumen, damaged basement membrane and decreased secretory cells in the hepatopancreas, disordered gill epithelial and pillar cells, and caused gill filament base vacuolation. Our study indicates that chronic ammonia stress impairs growth performance, tissue morphology, induces oxidative stress, and alters glucose and ammonia metabolism in juvenile oriental river prawns.
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    Dietary magnesium improves growth, antioxidant capacity, ammonia tolerance and intestinal health of juvenile freshwater prawn, Macrobrachium nipponense
    (Elsevier, 2022-11-15) Youqin Kong; Zhili Ding; Shanshan Wei; Changle Qi; Yan Liu; Yixiang Zhang; Samwel Mchele Limbu; Jinyun Ye
    The present study evaluated the effects of magnesium (Mg) on growth, antioxidant capacity, intestinal morphology and ammonia nitrogen resistance in juvenile oriental river prawn, Macrobrachium nipponense. Six semipurified diets (crude protein 401.1 g kg−1 diet; crude lipid 88.7 g kg−1 diet) containing Mg levels of 1.1, 1.4, 1.6, 2.1, 2.8 or 4.5 g kg−1 were formulated. A total of 1080 healthy juvenile prawns (0.151 ± 0.003 g) were randomly stocked into eighteen 300 L tanks (100 × 80 × 60 cm), each tank with 60 prawns. Each experimental diet was fed to prawns in triplicate twice daily (08:00 and 17:00) for eight weeks. After the feeding trial, 20 prawns from each tank were challenged with 37 mg/L ammonia nitrogen for 96 h, and the hemolymph, hepatopancreas and muscle of the rest of the prawns were collected. Final biomass was higher in prawns fed diets containing 1.6 and 2.1 g Mg kg−1 compared with those fed the diet with 1.1 g kg−1. Higher alkaline phosphatase (ALP) activity was obtained in the serum of prawns fed the 2.1 and 2.8 g Mg kg−1 diets. The highest activities of hepatopancreas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were observed in prawns fed 1.6–2.8 g Mg kg−1 of feed, and the lowest hepatopancreas malondialdehyde (MDA) concentrations and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were found in these treatment groups. Similarly, the prawns fed diets with 1.6 and 2.1 g Mg kg−1 had significantly lower MDA concentrations and cumulative mortality and higher antioxidant enzyme activities after ammonia nitrogen exposure. The intestinal epithelial cells were closely attached to the membrane in prawns fed the 2.1 g Mg kg−1 diet, but they were partly detached in those fed the 1.1 and 4.5 g Mg kg−1 diets. The minimum dietary Mg supplementations of juvenile M. nipponense were determined to be 1.6, 1.9, 2.0 and 2.2 g kg−1 feed, respectively, using broken-line regression analysis based on the final biomass, SOD, ALT and ALP.
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    Dietary thiamine modulates carbohydrate metabolism, antioxidant status, and alleviates hypoxia stress in oriental river prawn Macrobrachium nipponense (de Haan)
    (Elsevier, 2022-12) Dongsheng Zhou; Chengli Wang; Jinxian Zheng; Jianhu Zhao; Shanshan Wei; Yunfeng Xiong; Samwel Mchele Limbu; Youqin Kong; Fang Cao; Zhili Ding
    Hypoxia is one of the challenges in prawns aquaculture. However, the role of thiamine, which is a coenzyme in carbohydrate metabolism with antioxidant properties, in reducing hypoxia in prawns aquaculture is currently unknown. We investigated the effects of thiamine on antioxidant status, carbohydrate metabolism and acute hypoxia in oriental river prawn, Macrobrachium nipponense. One thousand eight hundred prawns (0.123 ± 0.003 g) were fed five diets (60 prawns each tank, six replicates per diet) supplemented with graded thiamine levels (5.69, 70.70, 133.67, 268.33 and 532.00 mg/kg dry mater) for eight weeks and then exposed to hypoxia stress for 12 h followed by reoxyegnation for 12 h. The results showed that, under normoxia, prawns fed the 133.67 or 268.33 mg/kg thiamine diet had significantly lower glucose 6-phosphatedehydrogenase, succinate dehydrogenase and phosphoenolpyruvate carboxykinase activities than those fed the other diets. Moreover, total antioxidant capacity (T-AOC) increased significantly when prawns were fed the 133.67 mg/kg thiamine diet. Superoxide dismutase (SOD) activity and malonaldehyde (MDA) content also increased significantly when prawns were fed the 268.33 or 532.00 mg/kg thiamine diet under hypoxia. And the significantly increased SOD activity and MDA level also observed in prawns fed 532.00 mg/kg thiamine under reoxygenation. Under normoxia, prawns fed the 70.70 or 133.67 mg/kg thiamine diet decreased the mRNA expressions of AMP-activated protein kinase-alpha (AMPK-α), pyruvate dehydrogenase-E1-α subunit (PDH-E1-α) and hypoxia-inducible factor-1s (HIF-1α, HIF-1β), but increased the mRNA expressions of phosphofructokinase (PFK) significantly. After 12 h of hypoxia, the energy metabolism related genes (AMPK-β, AMPK-γ, PFK, PDH-E1-α), hypoxia-inducible factor related genes (HIF-1α, HIF-1β) and thiamine transporter gene (SLC19A2) were up-regulated significantly in prawns fed the 133.67 or 268.33 mg/kg thiamine diets. After 12 h of reoxygenation, prawns fed the 133.67 or 268.33 mg/kg diet significantly decreased the SOD activity, MDA level and SLC19A2 mRNA expression compared with other diets. The optimum thiamine was 161.20 mg/kg for minimum MDA content and 143.17 mg/kg for maximum T-AOC activity based on cubic regression analysis. In summary, supplementing 143.17 to 161.20 mg/kg thiamine in the diets for M. nipponense improves the antioxidant capacity under normoxia and reduces the oxidative damage under hypoxia stress.