Browsing by Author "Nicklin, M. G."

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Recovery of Protein Antigens from Disulphide-Linked Immunosorbents Derived from Non-Avid Sera
    (Elsevier, 1974) Nicklin, M. G.; Stephen, Josephine K.
    Disulphide-linked antibody immunosorbents were prepared from non-avid antisera raised to human serum albumin, ovalbumin and lysozyme. The ease with which homologous antigen was released from such immunosorbents was compared with that from their fully avid counterparts. This was maximally demonstrated using acidic buffers of pH 4–5 and immunosorbent columns in which the disulfide-linked protei was mixed with Sephadex G-15 and placed on top of more Sephadex G-15. Conditions are described under which immunosorbent derived from non-avid sera adsorber and released homologous antigen 3–4 time more efficiently than their avid counterparts.
  • Thumbnail Image
    Item
    Solubilities of Protein-Antigen/Rabbit-Antibody Complexes as a Measure of Serum Avidity
    (Elsevier, 1973) Nicklin, M. G.; Stephen, Josephine K.
    Several immunizing schedules were examined for the production of rabbit antibodies to human serum albumin, ovalbumin and lysozyme, having lowered affinities for respective antigens.From those sera which contained precipitating antibodies, antigen/antibody complexes were made at optimum proportions at pH 7.0. The solubilities of such complexes were measured in buffers of constant ionic strenght (0·26) and of pH ranging from 2·5 to 10·0. The use of large quantities of alum-absorbed antigen, administered in small doses (schedule I) yielded sera, complexes derived from which were the most soluble at least extreme pH values. Sera raised with complete Freund's adjuvant (CFA) yielded the least soluble complexes. Administration of large quantities of alum-adsorbed antigens in two dosed produced sera whose complexes were similar to those of CFA sera. Sera taken early in the response to antigen and CFA yielded complexes similar to those of schedule I sera.Attention was focused on the effect of citrate buffer pH 4·0, in dissociating antigen/antibody complexes since at this pH value maximum solubility differences were observed between complexes derived from the greatest number of sera. Where technically possible antisera yielding complexes soluble at pH 4·0 were shown to yield dissociable complexes (by ultracentrifugation), to contain non-precipitating antibodies (by precipitin analysis), and to be non-avid (by the technique of Farr (1985)).