Browsing by Author "Mtolera, Matern S.P."

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    Assessment of Antioxidant Contents and Free Radical-Scavenging Capacity of Chlorella vulgaris Cultivated in Low Cost Media
    (MDPI, 2020-12) Mtaki, Kulwa; Kyewalyanga, Margareth Serapio; Mtolera, Matern S.P.
    The current study assessed antioxidants contents (total phenolics and total flavanoids, β-carotene and lycopene) present in Chlorella vulgaris (C. vulgaris) cultivated in low cost media and their free radical scavenging activities. Microalgae was cultured using Bold basal medium (BBM) as a control, 5% banana stem compost (BCM) and aquaculture wastewater supplemented with 1.0 g/L NPK (ANM). The free radical scavenging ability was analysed using 1,1-diphenyl-2-picrylhydrazyl assay. Cells grown on BCM resulted in higher (13.73 ± 0.121%) extraction yield than in other media. The phenolic (8.53 ± 0.10 mg/g GAE) and lycopene (0.29 ± 0.008 mg/g) content were highest in cells grown on BCM and BBM, respectively. Microalgae cultured in ANM displayed higher (547.023 ± 34.703 mg/g RE) flavanoid and β-carotene (2.887 ± 0.121 mg/g) content than in other media. Furthermore, cells cultivated in BCM showed highest (97.87 ± 0.088%) scavenging activity than in BBM. These results indicated that the BCM and ANM can be used as alternative to expensive synthetic media for antioxidant production in C. vulgaris.
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    Supplementing wastewater with NPK fertilizer as a cheap source of nutrients in cultivating live food (Chlorella vulgaris)
    (SPRINGER NATURE, 2021) Mtaki, Kulwa; Kyewalyanga, Margareth S.; Mtolera, Matern S.P.
    Introduction: The decline in fishery resources from the wild has led to an ever increasing focus on aquaculture in recent years. With increasing aquaculture of animal species, there is an increasing need for suitable microalgae in the production of these animals. However, cultivation of microalgae in expensive pure chemical media is one of the major challenges facing large-scale cultivation of microalgae. Purpose: The present study investigated the suitability of aquaculture wastewater (AWW) supplemented with NPK (nitrogen:phosphorus:potassium) fertilizer as a cheap source of nutrient to cultivate a microalga Chlorella vulgaris (C. vulgaris). Methods: C. vulgaris with an initial cell density of 0.8 × 106 cells/mL was batch cultured in AWW supplemented with NPK at 0.1, 0.5, 1.0 g/L and BBM for 20 days under laboratory conditions using 2000 mL Erlenmeyer flasks. The proximate composition, chlorophyll, minerals, and vitamins analysis of C. vulgaris biomass were done using standard analytical methods. Results: The highest values in optical density (4.872 ± 0.025), dry cell weight (2.858 ± 0.015 g/L), specific growth rate (0.2097 ± 0.0038 day–1), and biomass productivity (0.1701 ± 0.0007 g/L/day) were obtained in C. vulgaris grown in AWW + 1.0 NPK medium. The total chlorophyll, protein, lipid, and carbohydrate content of the microalgae biomass were in the range of 0.05–0.862%, 44.062–57.089%, 17.064–23.260%, and 15.217–21.896%, respectively. Furthermore, microalgae grown in AWW + 1.0 NPK showed good vitamin and mineral content compared to BBM grown alga. Conclusion: These findings indicated that the AWW + 0.1 NPK, AWW + 0.5 NPK, and AWW + 1.0 NPK are potential growth media for C. vulgaris cultivation and can replace the BBM medium, which is very expensive and less accessible to users.