Browsing by Author "Lyaruu, H. V. M."

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    Improving propagation success of D. Melanoxylon (African blackwood) in Tanzania (I)
    (Tanzania Journal of Science, 2012-03-15) Washa, W. B. A.; Nyomora, A. M. S.; Lyaruu, H. V. M.
    Dalbergia melanoxylon Guill & Perr is a plant with quality wood in the World and therefore is over harvested for timber while its regeneration capacity is very low. The propagation techniques such as tissue culture or use of mycorrhiza have not been investigated which instigated conduction of this study by investigating the presence of mycorrhiza that might be useful for its regeneration. Some 120kg of soil and 120 roots of D. melanoxylon from Kilwa, Kilosa and Babati were sampled to identify mycorrhiza related with regeneration of the species. Cleaning the tissues was done by soaking the root pieces in 1.79M KOH, and 0.1M Hcl. Staining was done using 0.05% Trypan blue and de-staining in 14:1:1 lactic acid: glycerol: water and mounting on slides for observation. Soil was soaked in water overnight and the mycorrhiza was separated using a stereo microscope at 50X. Separated mycorrhiza were incubated for observation using a compound microscope at 400X. Twenty six (26) ECM resembling Inocybe and Laccaria sp were isolated from the root pieces and 18 VAM resembling Glomus species were isolated from soil. It is recommended that future research should investigate proper inoculum types and time for inoculating the species in nurseries for propagation.
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    Improving propagation success of D. Melanoxylon (African blackwood) in Tanzania (II): Rooting ability of stem and root cuttings of Dalbergia melanoxylon (African blackwood) in response to rooting media sterilization in Tanzania
    (Tanzania Journal of Science, 2012-03-15) Washa, W. B. A.; Nyomora, A. M. S.; Lyaruu, H. V. M.
    Dalbergia melanoxylon is a plant with valuable wood in the world and therefore is over harvested for timber while its regeneration is very low. The propagation efforts by techniques such as tissue culture or mycorrhiza have not been investigated which instigated conduction of this study. Soil and cuttings were collected from Kilwa, Kilosa and Babati for rooting test, potting media and soil characterization. The results showed that fresh soil improved rooting characteristics while sterilized soil did not due to the presence of mycorrhiza in fresh soil compared to none in sterilized media. The overall rooting in fresh soil in non-mist propagator was higher, 100% for softwood and 37% for root cuttings while none rooted in the open nursery. The results revealed that there was no significant correlation between rooting and collection sites while cutting type and the soil type used significantly influenced rooting. Softwood cuttings that weighed less than 15g significantly increased rooting at (P < 0.05) compared to cuttings that weighed above 15g. It was recommended to propagate Dalbergia melanoxylon using softwood cuttings placed in a non-mist propagator and using fresh soil infected with mycorrhiza.