Browsing by Author "Kivaisi, A.K"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Identification of Mushroom Mycelia using DNA Techniques(2002) Muruke, M.H.S; Kivaisi, A.K; Magingo, Francis S.; Danell, E.The suitability of using DNA techniques in the determination of relatedness of mushroom fruiting bodies to isolated mycelia was examined. Nine isolates of edible mushroom mycelia of general Oudemansiella, Coprinus and Pleurotus were identified using fruiting bodies as references. Polymerase Chain Reaction (PCR) in conbination with Restriction Fragment Length Polymorphism (RLFP) analyses were carried out on fruiting bodies and mycelia of the isolates. The internally transcribed spacer region (ITS) and ribosomal RNA gene (rDNA) was amplified using ITS1 and ITS4 primers. The RLFP analysis was carried out on the regions amplified by PCR from fruiting bodies and the mycelia was established by looking at DNA fragment band sizes and patterns. Banding patterns and fragment sizes of DNA obtained from mycelia and their corresponding fruiting bodies were identical and characteristic for the species. Using this technique, it was possible to sort out a case of mistaken identity of Oudemansiella fruiting bodies, which were interchanged with another mushroom specimen during packing. The method is fast, accurate, and could be used for routine screening of edible mushrooms of Tanzania for taxonomical purposes. For the latter purpose, it is required that the RFLP database of taxonomically known species is in place.Item Performance of Pleurotus flabellatus on water hyacinth (Eichhornia crassipes) shoots at two different temperature and relative humidity regimes(2004-01) Kivaisi, A.K; Magingo, Francis S.; Mamiro, BEfforts are being made in Tanzania to promote mushroom cultivation, and identification of abundantly available plant biomass residues appropriate for growing mushrooms is part of the efforts. This study investigated the suitability of water hyacinth as a bulk substrate for growing a newly domesticated local oyster mushroom, Pleurotus flabellatus. The performance of the mushroom was investigated under ambient temperature and relative humidity (RH) regimes of 18-25/27-29 oC and 55-85/78-93%, respectively. The growth cycle of the mushroom was completed in 40 days with three and four flushes respectively. At the higher temperature and RH regime, the mushroom grew faster and the first flush was harvested at the 13th day after substrate inoculation with a Biological Efficiency (B.E.) of 84%, whereas the first harvest was done on the 19th day after inoculation at the lower temperature and RH regime with a B.E. of 53%. Substrate total fibre loss at the end of the growth cycle was in the range of 31-40%, and cellulose the most utilized fraction, decreased by 35-48%. The rates of fibre loss increased over time during the mushroom growth and were highest during the first and second flush during which about 80% of the total mushroom yield were obtained. Water hyacinth shoots proved to be a good substrate for growing the local oyster mushroom at ambient environmental conditions.