Mohammed, SalumMunissi, Joan J. E.Nyandoro, Stephen S.2016-06-262016-06-262016-02Mohammed, S., Munissi, J., & Nyandoro, S. (2016). Aflatoxin M1in raw milk and aflatoxin B1in feed from household cows in Singida, Tanzania. Food Additives & Contaminants: Part B, 9(2), 85-90. http://dx.doi.org/10.1080/19393210.2015.11373611939-32101939-3229http://hdl.handle.net/20.500.11810/2767Full text can be accessed at the following link http://www.tandfonline.com/doi/pdf/10.1080/19393210.2015.1137361Aflatoxin M1 (AFM1) contamination in raw milk from household cows fed with sunflower seedcakes or sunflower-based seedcake feeds was determined in 37 milk samples collected randomly from different locations in Singida region, Tanzania. Aflatoxin B1 (AFB1) contamination in sunflower-based seedcake feed was determined in 20 feed samples collected from the same household dairy farmers. The samples were analysed by RP-HPLC using fluorescent detection after immunoaffinity column clean-up. Recoveries were 88.0% and 94.5%, while the limits of detection (LOD) were 0.026 ng mL(-1) and 0.364 ng g(-1) for AFM1 and AFB1, respectively. Of the analysed cow's milk samples, 83.8% (31/37) contained AFM1, with levels ranging from LOD to 2.007 ng mL(-1), exceeding both the European Commission (EC) and Tanzania Food and Drug Authority (TFDA) limit of 0.05 ng mL(-1). Of the contaminated samples, 16.1% exceeded the Codex Alimentarius limit of 0.5 ng mL(-1). AFB1 was present in 65% (13/20) of the feed samples with levels ranging from LOD to 20.47 ng g(-1), 61.53% exceeding the TFDA and EC maximum limits of 5 ng g(-1) for complete dairy animal feed. The observed AFM1 and AFB1 contamination necessitates the need to raise awareness to dairy farmers in Tanzania to safeguard the health of the end-users.enAflatoxin M1Cow’s milkAflatoxin B1feedSingidaTanzaniaJournal Article, Peer Reviewed10.1080/19393210.2015.1137361