Patrick, FerdinandiMtui, GodlivingMshandete, Anthony M.Johansson, GunnarKivaisi, Amelia K.2016-05-172016-05-172009Patrick, F., Mtui, G., Mshandete, A.M., Johansson, G. and Kivaisi, A., 2009. Purification and characterization of a laccase from the basidiomycete Funalia trogii (Berk.) isolated in Tanzania. Afr. J. Biochem. Res, 3(5), pp.250-258.1996-0778http://hdl.handle.net/20.500.11810/2112A lignolytic basidiomycete fungus, Funalia trogii (Berk.), was isolated from decayed wood in coastal Tanzania and cultivated in submerged culture. Initially screened crude enzyme filtrate showed complete rhemazol brilliant blue - R (RBBR) decolorization 2,2’-azino-bis (3-ethylbenzthiazoline)-6-sulfonate and guaiacol oxidation after 7 days of incubation. The fungal filtrate had maximum laccase activity of 593 U/ml after 15 days of incubation. A laccase was purified by anion exchange and size exclusion chromatography to good purity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF). The isolated main component had a molecular weight of ca 58 kDa as determined by MS and an isoelectric point (pI) of 3.8. The optimal pH and temperature range for the purified laccase were 4.0 - 5.0 and 50 - 70 ºC, respectively, using 2, 6- dimethoxyphenol as a substrate.enFunalia trogiiLaccaseAnion exchange chromatographySize exclusion chromatographyElectrophoresisIsoelectric focusingJournal Article, Peer Reviewed