Lignin Peroxidase Production by Phanerochaete Chrysosporium Immobilized on Polyurethane Foam

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dc.contributor.authorNakamura, Yoshitoshi
dc.contributor.authorSawada, Tatsuro
dc.contributor.authorMtui, Godliving Y. S.
dc.contributor.authorKobayashi, Fumihisa
dc.contributor.authorKuwahara, Masaaki
dc.contributor.authorIto, Hiromichi
dc.date.accessioned2016-09-21T16:18:24Z
dc.date.available2016-09-21T16:18:24Z
dc.date.issued2005-02
dc.descriptionFull text can be accessed https://www.jstage.jst.go.jp/article/jcej/30/1/30_1_1/_articleen_US
dc.description.abstractProduction of lignin peroxidase by a white-rot basidiomycete, Phanerochaete chrysosporium, was investigated experimentally using polyurethane foam as a carrier of immobilized fungal mycelia. The immobilized cell culture using polyurethane foam as a carrier of mycelia yielded significantly greater lignin peroxidase activity than the conventional stationary liquid culture. The effects of operational conditions, such as the kind and number of polyurethane foam cubes, glucose concentration and temperature, on the lignin peroxidase production were examined. Addition of 0.05% Tween 80, 1 mM veratryl alcohol and 1 mM FeSO4-·7H2O greatly improved the production of lignin peroxidase up to 2,700 units/ml culture medium. The lignin peroxidase activity in this culture was about three times larger than that obtained from the culture cultivated in the absence of these additives. Step change incubation lowering the temperature from 37°C to 30°C over an incubation time of three days was carried out for the large scale production of lignin peroxidase, and this incubation gave the highest lignin peroxidase activity 3,800 units/ml culture medium.en_US
dc.identifier.citationNakamura, Y., Sawada, T., Sungusia, M.G., Kobayashi, F., Kuwahara, M. and Ito, H., 1997. Lignin Peroxidase Production by Phanerochaete Chrysosporium Immobilized on Polyurethane Foam. Journal of chemical engineering of Japan, 30(1), pp.1-6.en_US
dc.identifier.doiorg/10.1252/jcej.30.1
dc.identifier.urihttp://hdl.handle.net/20.500.11810/4058
dc.language.isoenen_US
dc.subjectPhanerochaete chrysosporiumen_US
dc.subjectLignin-Degrading Enzymeen_US
dc.subjectLignin Peroxidaseen_US
dc.subjectImmobilized Cell Cultureen_US
dc.subjectPolyurethane Foamen_US
dc.titleLignin Peroxidase Production by Phanerochaete Chrysosporium Immobilized on Polyurethane Foamen_US
dc.typeJournal Articleen_US
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