Cloning, tissue distribution and mRNA expression analysis of typeIcollagen alpha 1 gene from Chu's croaker (Nibea coibor)

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dc.contributor.authorHua Rong
dc.contributor.authorFan Lin
dc.contributor.authorLijun Ning
dc.contributor.authorKun Wu
dc.contributor.authorBaojia Chen
dc.contributor.authorJia Zheng
dc.contributor.authorSamwel Mchele Limbu
dc.contributor.authorXiaobo Wen
dc.date.accessioned2022-08-05T16:37:31Z
dc.date.available2022-08-05T16:37:31Z
dc.date.issued2022-05-25
dc.description.abstractThe demand for collagen has been increasing over years due to its wide application in food, cosmetics and biomedicine industries. The synthesis of collagen protein in fish depends on instructions provided by collagen, type I, alpha 1 (COL1A1) gene. However, cloning, tissue distribution and mRNA expression of COL1A1 gene in a gel-producing Chu's croaker (Nibea coibor) is currently unknown. This study cloned the cDNA of COL1A1 gene (GenBank accession number: MK641512) from six N. coibor fish. The distribution and mRNA expression pattern of COL1A1 was analyzed in eight tissues of N. coibor. The COL1A1 cDNA had a full length of 6130 bp and contained a 4344 bp open reading frame (ORF) encoding a polypeptide of 1448 amino acids. The homology of N. coibor COL1A1 amino acid had 98% similarity with Larimichthys crocea, indicating conservatism with other members in same family (Sciaenidae). The deduced polypeptide contained the same signal peptides, C-propeptide and N-propeptide domains, and triple helix domains, which are the characteristics of type I collagen in vertebrates. The mRNA of COL1A1 gene was expressed significantly higher in the spine of N. coibor than in all other tissues (P < 0.05), followed by swim bladder, skin and scales. The swim bladder had higher collagen and hydroxyproline contents than other tissues, followed by spine >, scales > and > skin (P < 0.05). Our study successfully cloned the COL1A1 gene from N. coibor for the first time. The COL1A1 gene contained all the features of collagen pro-α1(I) chain proteins, and shared high homology with other marine teleost. COL1A1 gene in N. coibor is highly expressed in spine and swim bladder, consistent with collagen distribution. Our study contributes to better understanding on collagen biosynthesis in N. coibor tissues for various industrial uses.en_US
dc.description.sponsorshipKey Program of Marine Economy Development (Six Marine Industries) Special Foundation of Department of Natural Resources of Guangdong Province (GDNRC [2020]076) project, the Team Project of Department of Education of Guangdong Province (2018KCXTD012) and Guangdong Province Basic and Applied Basic Research Fund—Provincial and Municipal Joint Fund (2021A1515110179)en_US
dc.identifier.citationHua Rong, Fan Lin, Lijun Ning, Kun Wu, Baojia Chen, Jia Zheng, Samwel Mchele Limbu and Xiaobo Wen (2022). Cloning, tissue distribution and mRNA expression analysis of typeIcollagen alpha 1 gene from Chu's croaker (Nibea coibor). Gene, 824: 146441. https://doi.org/10.1016/j.gene.2022.146441.en_US
dc.identifier.doihttps://doi.org/10.1016/j.gene.2022.146441
dc.identifier.urihttp://hdl.handle.net/20.500.11810/5878
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectNibea coiboren_US
dc.subjectCOL1A1 geneen_US
dc.subjectcDNA cloningen_US
dc.subjectCollagen synthesisen_US
dc.subjectChu’s croaker swim bladderen_US
dc.titleCloning, tissue distribution and mRNA expression analysis of typeIcollagen alpha 1 gene from Chu's croaker (Nibea coibor)en_US
dc.typeJournal Article, Peer Revieweden_US
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